Bacterial adherence on a polymeric coating applied on carbon steel

MONICA ZAPPONI; PABLO CASTRO; SANDRA G. GOMEZ DE SARAVIA; MAURO J. BANERA; SANDRO NICOLINO; VERÓNICA RODRIGUEZ BAUZADA; SILVIA E. RASTELLI; ALEJANDRO DI SARLI; SONIA BRUNO; MARISA R. VIERA ; CECILIA I. ELSNER

Buenos Aires

Congreso; IV Congreso de integridad y corrosión en la industria del petroleo y del gas; 2020

Instituto Argentino del Petroleo y del Gas

Microbialcontamination in the oil and gas industry results in a variety ofproblems in drilling fluids, hydraulic fracturing fluids, waterflooding, pipelines, and storage tanks. Microorganisms such assulfate-reducing bacteria (SRB), acid producing bacteria, and/orother halophiles can negatively impact asset integrity and reduce thequality and quantity of produced hydrocarbons. Organic coatings areused on the internal tube surface to prevent corrosion problems inservice. The aim of this study was to evaluate the performance of apolymeric coating applied on carbon steel in the presence of amicrobial consortium from an oil storage tank. The samples wereexposed to a batch culture of the microbial community in Postgate´sC medium in anaerobic conditions at 28 ±2 °Cfor 60 days. After that time, different techniques were applied toanalyze surface deterioration: Scanning Electron Microscopy (SEM),Electrochemical Impedance Spectroscopy (EIS), EpifluorescenceMicroscopy (EM). Furthermore, the planktonic and sessile microbialcommunities were analyzed by Next-Generation Sequencing (NGS) of 16SrRNA gene. The genetic analysis showed a shift in the bacterialcommunity along the experiment. SRB were predominant at thebeginning, diminishing their number with time. Bacteria belonging tothe Desulfovibrionales and Bacterioidales orders were the mostabundant in the biofilms formed on the samples. According to the EISresults, neither deterioration of the coating, nor corrosion of thecarbon steel was detected after the exposure to the cultures for 60days.Observación: El congreso se pospuso debido a la pandemia COVID-19p { margin-bottom: 0.25cm; direction: ltr; line-height: 115%; text-align: left; orphans: 2; widows: 2 }a:link { color: #0000ff }