?Improving Peak Capacity and Flexibility in On-Line Two Dimensional Liquid Chromatography by Flow-Splitting after First Dimension Column? (ponencia oral M. Filgueira)

FILGUEIRA, M.; CARR, P. W.; CASTELLS, C. B.; HUANG, Y.; WITT, K.

Orlando

Conferencia; Pittcon2012. Pittsburgh Conference on Analytical Chemistry and Applied Spectroscopy.; 2012

Pittcon

The use of a flow splitter after the first dimension in on-line comprehensive two dimensional liquid chromatography (LCxLC) has not received very much attention in comparison to their use in GCxGC. Splitting the flow after the first dimension column allows one to independently optimize both dimensions thereby increasing peak capacity. Sensitivity is somewhat diminished but this can be partially compensated by a larger injection or use of a wider column. Nonetheless any decreasein sensitivity is a small price to pay for the increased resolving power and system flexibility. Our results show that both the effective peak capacity and number of observed peaks nearly double at relatively short analysis times (15 minutes) when comparing systems with and without a flow splitter. A major advantage of the splitter is the increase in first dimension peak capacity resulting from the increase in the ratio of the first dimension gradient time to the overall first dimensionanalysis time.