Final Report IAEA: Radio-synthesis of protein-gold nanoparticles for theranostics applications

ALONSO, S. DEL V.; ACHILLI, ESTEFANIA; GRASSELLI, MARIANO; FLORES, CONSTANZA Y.

2018-04-02/2019-05-01

Nanotecnologia

Tecnologia sanitaria y curativa-Otros

Nanomedicines, especially in round shape particles or nanoparticles (NPs), are studied for application in the field of diagnosis and treatment of cancer. It is expected that this novel nanomaterials will have an improved degree of specificity to tumour cells through interaction with cell surface receptors. Noble metal nanoparticles have been the subject of studies for biology-oriented applications, especially gold nanoparticles for their outstanding properties under nanostructure shapes. In this report, it is described as a novel hybrid NPs (HNPs), containing a gold core and a multilayer of albumin stabilised by the radiation-induced crosslinking method. It is expected protein multilayer coating enhance the biocompatibility of the nanomaterial. HNPs are decorated with a DOTA-Bombesin synthetic peptide in order to address cancer cells which overexpress GRP receptors. The in-vitro specific interaction of these nanoconstructs to cancer cell lines were studied by flow cytometry analysis and confocal microscopy. It was demonstrated that HNP uptake is dependent of its decoration with the specific peptide and that the internalisation process is energy dependent.