IMBICE   05372
INSTITUTO MULTIDISCIPLINARIO DE BIOLOGIA CELULAR
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Is subcutaneous adipose tissue the most thermogenic depot? comparative analysis between two rodent models
Autor/es:
HARNICHAR, EA; ALZAMENDI, ANA; GIOVAMBATTISTA, ANDRÉS; GAMBARO, S; MIGUEL, IGNACIO; GIORDANO, A; PORTALES, ANDREA ESTEFANÍA; ZUBIRÍA, MARÍA GUILLERMINA
Reunión:
Congreso; LXV Reunión Científica de la Sociedad Argentina de Investigación Clínica.; 2020
Resumen:
It is known that cold exposure and β-adrenergic stimulation activate white Adipose Tissue (AT) browning. Also, it has been largely described that Subcutaneous AT (SCAT) from rodents has greater browning capacity. However, most studies were carried out in mice. Here, we compared the thermogenic capacity of different white AT depots in rats and mice. Male adult rats and mice were housed at Room Temperature (Rat-RT and Mice-RT) or at Cold Temperature (4°C for 7 days, Rat-CT and Mice-CT). SCAT and Abdominal AT pads (Epididymal (EAT) for mice and Retroperitoneal (RPAT) for rat) were dissected and processed for UCP-1 and PGC1α quantification (qRT-PCR). We found that both thermogenic markers were less expressed in SCAT from rats vs. mice, being even lesser upon cold exposure (2way ANOVA, interaction p