IMBICE   05372
INSTITUTO MULTIDISCIPLINARIO DE BIOLOGIA CELULAR
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Ghrelin system regulates neurons of the supramammillary nucleus
Autor/es:
AGUGGIA JULIETA P.; DANIELA CASSANO; URIARTE DONATI MAIA; MIRTA REYNALDO ; BARRILE FRANCO; DE FRANCESCO P NICOLAS; TOLOSA M. JOSE; GARCÍA GUADALUPE ; FERNANDEZ GIMENA; CORNEJO PAULA; SPRING VALDIVIA; PERELLO MARIO
Lugar:
Villa Carlos Paz (Córdoba)
Reunión:
Congreso; XXXIV Congreso Anual de la Sociedad Argentina de Investigación en Neurociencias; 2019
Institución organizadora:
Congreso de la Sociedad Argentina de Investigación en Nuerociencia
Resumen:
Ghrelin is a stomach-derived hormone that acts via the growth hormone secretagogue receptor (GHSR) and regulates a variety of physiological functions. GHSR is expressed in the supramammillary nucleus (SuM), a hypothalamic area involved in behaviors associated with food intake, food-reward and novelty. However, the effects of ghrelin in the SuM are uncertain. Here we used mice, in which the enhanced green fluorescent protein (eGFP) is expressed under the control of the GHSR promoter (GHSR-eGFP mice), to gain neuroanatomical and functional insights of the GHSR neurons of the SuM. First, we validated the GHSR-eGFP mice as a reporter model for GHSR neurons in the SuM. The aim of this work was investigated the ability of ghrelin to reach and activate SuM. We used subcutane or intraventricular injections of a fluorescein-ghrelin (F-ghrelin) to test ghrelin`s ability to reach SuM nuclei. We also tested SuM responsiveness to ghrelin using immunohistochemistry (IHC) against the marker of neuronal activation c-Fos in different conditions (fasted, calorie restricted treatment). Finally, we test if SUM eGFP+ cells were dopaminergic or GABAergic neurons