Spexin, friend or foe? Study of the role of Spexin during thermogenesis of white adipose tissue.

ZUBIRÍA, MARÍA GUILLERMINA; HARNICHAR, EA; GAMBARO, SABRINA ELIANA; PORTALES ANDREA; GIORDANO, A; GIOVAMBATTISTA, ANDRÉS

Mar del Plata

Congreso; A LXIII Reunión Científica de la Sociedad Argentina de Investigación Clínica.; 2019

Spexin (SPX) is a novel adipokine related to appetite and weight control, glucose homeostasis, and lipidic metabolism. Plasma levels are reduced in obese and type II diabetes patients. Recent reports showed that SPX inhibits white adipogenesis and stimulate lipolysis. Our aim was to study SPX effects during the thermogenic process of white adipose tissue (AT). This process has been widely proposed as a possible therapy for obesity. C57BL/6J male mice were treated or not with SPX for ten days (ip. 29 µg/kg/day; CTR and SPX). At day 3 animals were divided and a group was kept at room temperature (RT) and the other at 4°C (CTR-C and SPX-C). Body weight and caloric intake were recorded every day. At the end of the experiment, plasma was collected for metabolic parameters measurement and Epididymal AT (EAT) and Inguinal AT (IAT) were dissected for quantification of thermogenic markers (UCP1, PGC1a and COX8b) by qPCR. Total caloric intake was increased upon cold stimulation (P