Pre-puberal androgen depletion improves the white adipose tissue browning

GIORDANO, A; HARNICHAR, EA; SPINEDI E; ZUBIRÍA, MARÍA GUILLERMINA; GIOVAMBATTISTA, ANDRÉS

Mar del Plata

Congreso; A LXIII Reunión Científica de la Sociedad Argentina de Investigación Clínica.; 2019

It is known that cold exposure induce the generation of beige adipocytes in white adipose tissue (AT) depots. Previously, we have described that the addition of androgens (A) on a mature beige adipocyte culture inhibits its thermogenic capacity. Now, we aim to study if in vivo absence of A affects the in vitro beige adipocyte generation and browning potential of white AT after cold stimulation. For this, we use the following groups of S-D male rats: controls (CTR), orchidectomized bilaterally at 27 days of age (ODX) and pair fed controls (CTR-PF, same ODX group caloric intake). In the first assay, adipocyte precursor cells (APCs) were isolated from inguinal AT (IAT) and retroperitoneal AT (RPAT) from different groups and were cultured with a pro-beige cocktail. At day 8, beige adipocyte markers (UCP-1, PGC1α, and PRDM16) were measured by RT-qPCR. We found an increase in UCP-1 (p