IMBICE   05372
INSTITUTO MULTIDISCIPLINARIO DE BIOLOGIA CELULAR
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
IN VIVO AND IN VITRO DEXAMETHASONE TREATMENT ENHANCES WHITE ADIPOCYTE PRECURSOR CELLS COMPETENCY
Autor/es:
SPINEDI E; ZUBIRIA G; GIOVAMBATTISTA A; GIORDANO AP
Lugar:
Mar del Plata
Reunión:
Congreso; LXIV Reunión de la Sociedad Argentina de Investigacion Clinica (SAIC).; 2019
Resumen:
Dexamethasone (DXM) is asynthetic GC widely recognized as an adipogenic inducer in both, in vivo and in vitro models. However, there is scarce information about the DXMrole in adipocyte precursor cells (APC) before differentiation induction. Ouraim was to evaluate the DXM effect on APC number and competency, using in vivo and in vitro approaches. Therefore, we ip injected two groups of adult male rats for 2 or 7 seven days with either DXM(30 mg/kgof weight, DXM-d2 and DXM-7d, respectively) or vehicle (CTR-2d and CTR-7d).Retroperitoneal adipose tissue (RPAT) was dissected and stromal vascular fraction(SVF) cells were isolated for Fluorescence-Activated Cell Sorting (FACS) analysis(APC profile: CD34+/CD45-/CD31-) andexpression of competency markers (PPARg2 and Zfp423). Two days after DXM treatment PPARg2 andZfp423 expression levels were increased (P