IMBICE   05372
INSTITUTO MULTIDISCIPLINARIO DE BIOLOGIA CELULAR
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Changes in Mesenchymal Stem Cells derived exosomes caused by UV-C radiation
Autor/es:
ALESSANDRA NORRIS; LUCÍA MORO; SANTIAGO GABRIEL MIRIUKA; MARÍA CELESTE BIANI; PAULA BUCCI; ALEJANDRO LA GRECA; CARLOS DANIEL LUZZANI; ANTONELLA LOMBARDI; DANIELA MARTIRÉ GRECO; JORGE MONTANARI
Lugar:
Mar del Plata
Reunión:
Congreso; S A I C . S A F E . S A B . SAP 2 0 1 9; 2019
Resumen:
Exosomes are extracellular vesicles ranging from 30 nm to 150 nm ​that originate from endosomal pathway. They contain proteins, lipids and nucleic acids, suggesting a possible role in cellular communication. In our study, we sought to elucidate the effect genomic damage provoked by UV-C may have on exosomal secretion of mesenchymal stem cells derived from induced pluripotent stem cells (iPS-MSC). Here, iPS-MSC were irradiated with three different UV-C intensities (0,001 J/cm2, 0,01 J/cm2 and 0,1 J/cm2) and the expression of genes involved in the exosomal pathway was evaluated by RT-qPCR. TSAP6, ALIX, Syntenin, Rab27a and P21 did not show significant difference in the three conditions. Given these results, exosomes secreted by irradiated and not irradiated IPS-MSC were isolated from conditioned media using a Size Exclusion Chromatography column and the concentration of extracellular vesicles was measured by Tunable Resistive Pulse Sensing. No significant differences were shown between conditions. To assess if there is a change in intercellular communication due a distinct exosome content, functional assays were performed. A wound Healing assay showed that cells incubated with not irradiated IPS-MSC derived exosomes had significant more wound closure than those incubated without exosomes, and the effect is lost when cells are incubated with irradiated IPS-MSC derived exosomes. We were able to verify that these results are not due to changes in cell replication, as analysis with propidium iodide showed no differences in cell cycle between the conditions. Taken together, our results suggest that exosomal secretion could be altered due to genomic damage caused by UV-C. While genes of exosomal pathway and the number of total exosomes secreted by irradiated and non irradiated IPS-MSC does not show differences, changes are evidenced in exosomal function. We hypothesize that there may be a distinctive exosome composition between the conditions that remains to be explored.