Protein nanoparticles as carriers of natural antioxidants

CANDIDO SOFIA; MARTINEZ LUIS; J. C. MORENO; ALVIRA, F.C.; DEL ALONSO, S.V.

San Luis

Congreso; XVLIII Reunio Anual de la Sociedad Argentina de Biofísica; 2019

Sociedad Argentina de Biofísica

We have conducted physically andrheological characterization of a protein nanocarrier (bovine serum albuminnanoparticles) to encapsulate antioxidants of natural origin coming fromblueberries (Vaccinium Myrtillus). The blueberries are rich in anthocyanin (A).The encapsulation was done with the aim of obtaining a stable formulation of NPBSA + A that can be useful to enrich food or to be injected into the body. Dueto BSA NP is formed by an unknown number of individual BSA molecules,computational simulations were performed to estimate this number.BSANPs are obtained by a chemical method and stabilized by Gamma irradiation.Antioxidants are obtained by liquid-solid extraction from blueberries. The nanoformulationwas studied by, Dynamic Light Scattering (DLS), potential Z, electrontransmission microscopy, rheology, UV-VIS and fluorescence spectroscopy atthree different temperatures, 8 °C (storage temperature), 25 °C (environmenttemperature), 37 °C (physiological).Our results show that the BSA NPsobtained by elution in a molecular exclusion column has a hydrodynamic radiusof 28 +/- 5 nm and is composed of approximately 183 BSA molecules. Dependingstrongly on the temperature.It was possible to study the interaction of NPBSA + A and discriminate the active site where it occurs (Sudlow I and/or II),by fluorescence. From the rheological tests, it is observed that for all threetemperatures the samples tend to a flow behavior of the Newtonian type and thatthere is a correlation between viscosity and temperature values. Themorphological changes observed by TEM corroborate the relationship of viscosityvalues with aggregate formation.