IMBICE   05372
INSTITUTO MULTIDISCIPLINARIO DE BIOLOGIA CELULAR
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Isolation and characterization of different ASIP transcripts expressed in the skin of llama
Autor/es:
NOCELLI C; RENIERI C ; M. ANELLO, ; RODRIGUEZ C C ; ANELLO M, DAVERIO S, ARBELETCHE VIDAL RIOJA L, DI ROCCO F; ,M.S.DAVERIO, ; VIDAL RIOJA ARBELETXCHE L.
Lugar:
ORURO
Reunión:
Congreso; VIII Congreso Mundial en Camelidos Sudamericanos; 2018
Institución organizadora:
Universidad Tecnica de Oruro
Resumen:
? Isolation and characterization of different ASIP transcripts expressed in the skin of llamaABSTRACT: The llama (Lama glama) presents a wide variety of color phenotypes. It is known that the locus responsible for colored phenotypes is the Agoutí locus. Moreover, Agoutí signaling protein gene (ASIP) controls the distribution of pigments using of different promoters and alternative non-coding first exons. The aim of this work was to isolate and characterize ASIP transcripts in the skin of llama. To do so, skin biopsies were taken and total RNA was extracted. PCR-RACE was used to amplify mRNAs corresponding to ASIP with their 5´UTR ends. Then, these products were cloned and sequenced. So far, three different ASIP transcripts were isolated and characterized. They presented similar structure and different degrees of similarity with ASIP transcripts described in other mammalian species. In addition, a common sequence of 10 bp preceding the AUG was observed in all three of them. We are currently continuing with this study since it is possible that there are more ASIP transcripts not yet isolated. Afterwards, we plan to complete the work with an expression analysis and association studies between the expression levels of the transcripts and coat color phenotypes. These results will be useful to understand the role ASIP plays in the pigmentation mechanisms of the llama.