IMBICE   05372
INSTITUTO MULTIDISCIPLINARIO DE BIOLOGIA CELULAR
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Multi-walled carbon nanotubes cause different developmental and organ toxicity on Zebrafish determined by their dimension
Autor/es:
CZARNOWSKI IAN; IGARTÚA DANIELA EDITH; PRIETO MARIA JIMENA; MARTINEZ CAROLINA SOLEDAD; ALONSO SILVIA DEL VALLE
Lugar:
Cuernavaca
Reunión:
Simposio; V Latin American Zebrafish Network Course and Symposium; 2018
Resumen:
Background. Carbon nanotubes are graphene sheet structures with a striking mechanical strength, chemical stability, and exceptional electrical and thermal conductivity. Its application in constantly growing, ranging from printer inks to drug delivery systems. Since the physicochemical properties of the compounds vary when they are produced at nanoscale, and that the environmental systems are dynamic, it is not possible to predict the hazards associated with the release of nanomaterials into the environment both for human and ecosystem health. Objectives. Carry out a comprehensive analysis of the toxicity of water and effluents by studying the behavior of new technologies in ecosystems; in this work, carbon nanotubes were studied. Methods and Results. Multi-walled carbon nanotubes (MWCNT) of different dimension were analyzed by scanning electron microscope (SEM). MWCNT A was short and width, MWCNT B was large and thin. Zebrafish were used as biomarkers of toxicity due to their small size, high fertilization rate and a rapid external development of transparent embryo. Tests done were acute toxicity (mortality), teratogenicity (hatching rate and morphology), general developmental anomalies and tissue-specific toxicity (morphology and functioning of brain, heart, and liver). Zebrafish embryos were incubated with 0.005-50 ppm MWCNTs, then mortality, hatching rate, and morphology were evaluated. Zebrafish larvae were incubated with 0.005-50 ppm MWCNTs, next morphology (bent spine, jaw malformation, head opacity, liver opacity, yolk opacity, small head, tail malformation, and uninflated swim bladder) and functioning of brain and heart were analyzed. We observed that MWCNTs were non lethal or teratogenic at the concentrations evaluated. MWCNT A caused a depression of the locomotor activity of larvae, indicating an alteration of the central nervous system; however, no morphological changes were observed. MWCNT B caused malformations in larvae and alteration of cardiac rhythm. Conclusions. Results were different for both carbon nanotubes, indicating a potential dissimilar toxicity between MWCNTs, highlighting the importance of dimension information of nanomaterials for the in vivo toxicity.Supported by PUNQ 1388/15, PUNQ 1076/15, Alonso-PIP/14, Montanari-PIP/16, and PICT CABBIO 0511/14.