ROLE OF CDK4 IN THE REGULATION OF WHITE ADIPOSE TISSUE BROWNING

ZUBIRIA GUILLERMINA; MIGUEL IGNACIO; GIOVAMBATTISTA , ANDRÉS; PORTALES ANDREA; GAMBARO, SABRINA

Mar del Plata

Congreso; LXII REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA (SAIC); 2017

SAIC

Cyclin-dependent kinase 4 (CDK4), has been involved in theadipose tissue (AT) biology through the control of white adipocytedifferentiation and insulin response. Previously, we showed that itsinhibition in vitro induces adipocyte precursor cells (APCs) determinationtowards the beige adipocyte lineage. In this study, we aimedto evaluate if CDK4 could regulate the browning of white AT in vivoand in vitro. To this end, C57BL/6J male mice were gavaged withCDK4 inhibitor (Palbociclib (PAL), 50mg/Kg) or vehicle (CTR) for10 days. Caloric intake and body weight were recorded every dayand glucose tolerance test (GTT) was performed on day 8. After thetreatment, mice were euthanized and plasma, epididymal AT (EAT)and liver samples were collected. EAT was weighted and processedfor APC isolation, histology and qPCR analysis. PAL treatment didnot induce changes in caloric intake or GTT, however, we observeda significant decrease in body weight and plasma and liver triglycerides(p