Effect of estradiol on the adipogenic process in retroperitoneal and inguinal adipose tissue of female rats.

MARTÍN MF; SPINEDI E; ALZAMENDI A; GIOVAMBATTISTA A; MIGUEL I; REY MA

CABA

Congreso; LXII Reunion de la Sociedad Argentina de Investigacion Clinica (SAIC); 2017

Sociedad Argentina de Investigaciones Clínicas (SAIC)

The mechanism whereby estradiol (E2) regulates adipose tissue (AT) development is still unclear. We previously showed beneficial effects of the lack of endogenous E2 (ovariectomized rats; OVX) on retroperitoneal AT (RPAT) function. Moreover, an in vitro E2-dependent decreased adipogenic potential was found in RPAT adipocyte precursor cells. Our aim was to assess the role of E2 deficiency on RPAT and inguinal AT (IAT) pads from female rats. S-D rats were divided into three groups: sham operated (CTR), OVX and OVX pair-fed (OVX-PF); OVX was preformed on prepubertal age (27 day-old). Food availability for CT and OVX rats was ad libitum, while for OVX-PF food provided was in the same amount as CTR rats ate. Food intake and body weight (BW) were recorded daily between surgery and experimental day (60 day-old). Trunk blood was collected to measure the plasma levels of several metabolites. RPAT and IAT pads were dissected-out, weighed and histological analyses were performed. Stromal vascular fraction (SVF) from both tissues was isolated to study the mRNA levels of AT functional genes (qPCR). OVX induced a decrease in RPAT pad mass (absolute and in % of BW; OVX P