Chronic endogenous glucocorticoid excess and white adipose tissue browning.

GIOVAMBATTISTA A; MARTÍN MF; SPINEDI E

CABA

Congreso; LXII Reunion de la Sociedad Argentina de Investigacion Clinica (SAIC); 2017

Sociedad Argentina de Investigaciones Clínicas (SAIC)

Glucocorticoids (GC) have different effects on white adipose tissue (WAT) and inhibit brown adipose tissue (BAT) thermogenesis; however, no whether GC affects browning or not remains unclear. Our aim was to study the effect of chronic endogenous GC excess on browning activity of epididymal WAT (eWAT) stromal vascular fraction cells (SVFs) from 90 day-old, neonatally-treated, monosodium L-glutamate (MSG) male S-D rats. MSG and litter-mate controls (CTR) eWAT pads were dissected and processed for histological appearance and gene (qPCR) analysis. Then, isolated eWAT SVFs were processed for gene expression analysis or cultured up to reach confluence. Confluent cells were 3 day-differentiated with a classical pro-browning cocktail. On differentiation day 8, cells were stimulated with 10µM forskolin (4 h) or not, and cell UCP1 mRNA levels were quantified. Body weight and food intake were lower (p