Proton channel inhibition alters cell proliferation and cell cycle in breast cancer cells

VENTURA, CLARA; MARTÍN, PEDRO; MILESI, MARIA VERÓNICA; ENRIQUE, NICOLÁS; COCCA, CLAUDIA; ASUAJE, AGUSTÍN; NUÑEZ, MARIEL

CABA, Argentina

Congreso; Reunion Conjunta de Sociedades de Biociencias; 2017

Metabolic reprogramming of cancer cells conduces to a high pro-duction of acidic substances which must be extruded to maintain cell viability. Here, we studied the effect of 2-(6-chloro-1H-benzimid-azol-2-yl)guanidine (ClGBI), an inhibitor of the proton channel Hv1, on proliferation and cell cycle of tumorigenic (MDA-MB-231 and MCF-7) and non-tumorigenic (MCF-10A) human breast cell lines. Concentration-response curve (0-20μM) and time-course (10-48h) were assayed to study cell proliferation (clonogenic assay), cell via-bility (MTT), cell cycle (flowcytometry) and mitotic cells (immunofl-orescence using DAPI and anti-α-tubulin antibody). Results: ClGBI reduced the clonogenicity of MDA-MB-231, MCF-7 and MCF-10A cells in a concentration dependent way (IC50: 3.4±0.2, 2.6±0.7 and 6.0±0.4 μM, respectively), getting the maximal inhibition at 10μM (p