IMBICE   05372
INSTITUTO MULTIDISCIPLINARIO DE BIOLOGIA CELULAR
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
HYBRID PROTEIN-GOLD NANOPARTICLES WITH BOMBESIN FOR IMPROVING CELL DELIVERY
Autor/es:
GILBERTO WEISMULLER; ESTEFANIA ACHILLI; MARIANO GRASSELLI; BEATRIZ PATRICIO CARVALHO; SILVIA ALONSO; CONSTANZA Y. FLORES
Lugar:
Sao Pablo
Reunión:
Congreso; 14° Congresso da Sociedade Latino Americana de Biomateriais, Orgãos Artificiais e Engenharia de Tecidos ? SLABO; 2017
Institución organizadora:
Sociedad Latina de Biomateriales
Resumen:
The use of nanotechnology in medicine, also called nanomedicine, is based on the preparation of nanostructures such as nanoparticles (NPs), with particular physicochemical characteristics which can be selected. They can be targeted to specific tumour tissues and modulate their pharmacokinetics, pharmacodynamics and enhanced intracellular activation properties. These characteristics depend on the size and surface properties of the NPs.Albumin-based NPs can be designed for a variety of uses; among them, the ones that stand out are nanovehicles in a drug delivery system. Such choice relies in on the fact that Albumin is a protein with high biocompatibility, and is biodegradable and non?antigenic as well as malleability. Therefore, Albumin is an excellent material to be used for biocompatibility of inorganic materials such as gold NPs (Au-NPs). In addition, NP targeting for molecular recognition of anomalous cells could improve the drug delivery efficiency. In this work are shown a preparation method of core/shell gold/Albumin NPs (Au/Alb-NPs) by desolvation method and crosslinking by ionizing radiation. Preparation of NPs containing an inorganic core such as gold can be very useful for NP tracking and detection. These Au/Alb-NPs are further decorated with a Bombesin-like peptide for prostate tumoral cell recognition. Extensive characterization of the nanoconstructs has been done by LDS, FT-IR spectroscopy, zeta potential and AFM microscopy analysis. Hybrid NPs in the range of 60-80 nm have been achieved.The nanostructured material was used to study the cellular uptake to PC3 prostate cancer cells. Multilayer Albumin coating shows higher cells uptake than the monolayer protein coated Au-NPs. Further studies should be done to study the cancer cell delivery in vivo.These materials showed promising characteristics to be used as intracellular delivery carrier of antitumoral and/or diagnosis agents.