IN-VITRO UPTAKE OF HYBRIDS GOLD/PROTEIN NANOPARTICLES BY TUMOR CELLS.

SILVIA DEL VALLE ALONSO; ACHILLI, ESTEFANIA; MARIANO GRASSELLI; CONSTANZA FLORES; FACUNDO TEMPRANA

Bernal

Simposio; II Simposio de Jovenes Biofisicos; 2017

Sociedad Argentina de Biofisica

Functionalization of gold nanoparticles (Au-NPs) surface with different biomolecules has been used for several decades in rapid diagnostics tests. In last years, Au-NPs have been demonstrated can be used in therapy, imaging and delivery applications. This nanomaterial platform could combinethose to generate theranostic nanoparticles.In order to localize the theranostic effect onto some specific cells and focus the therapy, NPs targeting is mandatory by using specific peptides, organic molecules or proteins. In addition to that, it is necessary to reduce non-specific interactions with other proteins which strongly reduce the NPs enrichment in the target. It has been recently recognized that the surface of the NPs and their interaction with serum proteins (´corona effect´) has a pivotal role in the final destination in the body. Therefore, the coating process became in a strategic issue to develop functional nanomaterials.In this context, the aim of this work is to prepare and characterize gold/albumin NPs (Au/Alb-NPs) decorated with DOTA-bombesin (DOTA-BB). The Alb coating is useful for reduction of the corona effect and Bombesin to target GRP receptor, present in several tumor cells, such as prostate and breast cancer. DOTA chelator will transport radionuclides.The Au/Alb/BB-NPs were characterized by UV-visible, dynamic light scattering (DLS), atomic force microscopy (AFM). Determination of DOTA-BB onto surface NPs is performed by MALDI TOF/TOF and by ICP-AES. Specific NPs uptake has been study by Cytometric Flow Analysis comparing NPs prepared by monolayer physisorption and our method decorated with the specific peptide onto PC-3 cell culture.Decorated hybrids NPs have less unspecific adsorption and better receptor recognition than the monolayer coated NPs.