IMBICE   05372
INSTITUTO MULTIDISCIPLINARIO DE BIOLOGIA CELULAR
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Development and characterization of polymeric nanoparticle
Autor/es:
B. F. C. PATRICIO; S. D. V. ALONSO; P. M. BISCH; C. MARTINEZ; A. LOPES; M. J. PRIETO; T. BARBOZA; G. WEISSM¨ULLER
Lugar:
Edimburgo
Reunión:
Congreso; 19th IUPAP Congress and 11th EBSA Congress; 2017
Resumen:
Nanotechnology aim to solve several pharmacological problems.To guarantee the success of nanoparticles (NP) it isimportant to ensure homogenous particle size, surface hydrophilicityand prolonged circulation time. This work aimedto produce and evaluate PLA/PVA and PEG/PLA/PVA NPwith different size and surface properties and evaluated itby in vivo test. The NP were produced by double emulsionmethod with changes in both power and sonication timeto obtain NP with different sizes. Using the Zebrafish WTmodel (CE-UNQ 2/2014) it was possible to observe that thePLA/PVA NP were more cardiotoxic than those pegylated.Furthermore, hepatotoxicity and neurotoxicity were higherin large pegylated NP. Finally, both NP were labeled with99mTc and injected into Wistar rats (CEUA 086/15). Itwas obtained gamma camera images and the organs activitywere measured. It was possible to define that pegylation decreasedthe uptake of the lager NP by the liver, but do notchange the uptake for the smaller ones. The non-peguilatedNP accumulated in the lungs. In this work, it was possible todetermine the better conditions for production of NP. Also, itallows the understanding of the toxicity and biodistributionof the nanoparticles at in vivo models.