Dendrimers Efficiency as a Drug Delivery System for Antiepileptic Drugs: A Biophysical Study

MARTINEZ CS; ALONSO SDV; IGARTÚA DE; PRIETO MJ

Edimburgo

Congreso; 19th IUPAB congress; 2017

Institute of Physics

The use of Dendrimers (D) as nanotechnology tools in medicine has soared up in recent years. The PAMAM D generation 4.0 and 4.5 are specially known as efficient drug delivery systems. Inthis work the use of these D are studied to enhance the solubility and delivery of the antiepileptic drug Carbamazepine (CBZ). The CBZ also has potential uses for the treatment of numerousneurodegenerative diseases, for example for Alzheimer Disease due to its activity as autophagy enhancer. However, it has low solubility in aqueous media, inefficient pharmacokinetic profilesand multiple side effects, so it is important to avoid widespread distribution. The biophysical characterization of D-CBZ complexes included time stability, endurance to a lyophilizationprocess, D-CBZ interaction by FTIR, and kinetic release profile of the drug by micro-dialysis. The complexes obtained resulted in drug delivery systems were the ratio CBZ:D was 20:1, whichpresented stability against the lyophilization process, had a controlled drug release, where interaction was mainly based on hydrophobic forces. In addition, the complexes were tested ex vivo(human red blood cells), in vitro (Neuro2A cell line), and in vivo (zebrafish larvae ). No hemolytic effect was observed ex vivo. Contrary to this, there was a toxic of the CBZ in both in vitro andin vivo studies, but not of the DG4.5-CBZ complex. The D as a nanovehicle in this study resulted in an efficient drug delivery system to bind CBZ. The potentiality relays on their ability tosignificantly prevent the toxic effects of the drug in both in vitro and in vivo models.