IMBICE   05372
INSTITUTO MULTIDISCIPLINARIO DE BIOLOGIA CELULAR
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
. Neural stem cells differentiation and outgrowth mediated by trophic factorsaction inside a 3D bioassay of collagen gel.
Autor/es:
CRUZ GAITÁN, ANA M; SCICOLONE, GABRIEL; FERNÁNDEZ, JIMENA P; CARRI, NÉSTOR GABRIEL; SPELZINI, GONZALO
Lugar:
Ciudad Autónoma de Buenos Aires
Reunión:
Congreso; 2nd FALAN Congress 2016; 2016
Institución organizadora:
Federation of Latinamerican Neuroscience Societies, Sociedad Argentina de Investigación en Neurociencias
Resumen:
NEURAL STEM CELLS DIFFERENTIATION AND OUTGROWTH MEDIATED BY TROPHIC FACTORS ACTION INSIDE A 3D BIOASSAY OF COLLAGEN GELSubventricular Zone from mammals has stem cells but little is known about its proliferation and differentiation. The aim of this study was analyze Corpus Striatum(CS) cells under basal conditions(BC) (DMEM-F12, bFGF, hEGF, B2, 37ºC, 100% hum, and 5% CO2, and subsequent trophic stimulation (FGF9, Noggin or GDNF) to induce differentiation and outgrowth. Cells obtained from CS of E14 rat embryo were cultured in suspension under BC; after 7DIV an aliquot of neurospheres was placed into 3D bioassay of collagen gel to submit trophic factors action; after 48h of 3D culture, we fixed and measured the length and amount of produced neurites. The expression of markers for proliferating cells (Ki67), progenitors (Nestin), Neuronal (TubIII and C-FOS), and glia (GFAP) were analyzed by immunocytochemistry. As results, CS cell culture showed a clear neurospheres formation, wich still is a main characteristic of Neural Stem Cells. In the 3D bioassay trophic stimulated cells generated a large number of long neurites, founding differences respect to no treated ones. Noggin produces a major stimulation over the differentiation and outgrowth. The expression of antigens was positive for all antibodies used. The 3D assay has a capacity to permit differentiation, proliferation and outgrowth that could be identified by the immune-profile as progenitors, neural and glial cells, also showed high reproducibility and resolution for tracing neural and glial outgrowth in short culture time.