Variation in Mitochondrial Genome and Telomere Length Correlates with DNA Methylation in Human Breast Cancer

CANÉ L; LONGARZO L; CÁLCENA E; PELTOMÄKI P.; BOLZÁN AD; PAVICIC WH; LONGARZO L; DEBANDI M; PELTOMÄKI P.; RICHARD SM; PAVICIC WH; DEBANDI M; RICHARD SM; CANÉ L; CÁLCENA E; BOLZÁN AD

Congreso; LXI Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC); 2016

Nuclear genes methylation plays an important role in human tumorigenesis. Many Tumor Suppressor Genes (TSG) and miRNA genes have associated CpG islands, suggesting epigenetic regulation of their expression. Mitochondrial DNA copy number variation (mtDNA-CN) and telomere length (TL) alteration could be involved in methylation changes on nuclear genes. We investigated 62 tumoral/non-tumoral adjacent (T/N) paired samples from Argentinean breast cancer patients. Methylation status at their promoters, was analyzed by MS-MLPA method on 24 TSGs, 10 miRNAs and the surrogate marker for global methylation LINE-1. The CIMP (CpG island methylator phenotype) condition was also evaluated. TL and mtDNA-CN variations were determined by qPCR method. We found a significant mtDNA-CN decrease in 63% of the samples by comparing T with the corresponding N tissue (average decrease = 24%, p