IMBICE   05372
INSTITUTO MULTIDISCIPLINARIO DE BIOLOGIA CELULAR
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
PREVENTIVE EFFECT OF A PROBIOTIC ADMINISTRATION ON THE METABOLIC ALTERATIONS CAUSED BY A FRUCTOSE RICH DIET INTAKE
Autor/es:
ZUBIRIA GUILLERMINA; RUMBO, MARTÍN; CARASI PAULA; GIOVAMBATTISTA , ANDRÉS; GAMBARO SABRINA ELIANA; SERRADELL MARIA DE LOS ANGELES
Lugar:
Mar del Plata
Reunión:
Congreso; LXI REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA; 2016
Institución organizadora:
SAIC
Resumen:
There is large evidence that gut microbiota modulates metabolic disorders associated Yith obesity. 9e evaluated the preventive effect of a probiotic Lactobacillus administration on the metabolic alterations caused by a fructose rich diet F4&. Four different SYiss mice groups Yere studied n¬ , CT4 mice Yere given tap Yater, F4& mice Yere given Y/v fructose in the drinMing Yater, and tYo similar groups that received by gavage 108 CF7 of .actoDacillus Mefiri C+&CA every hs CT4 LM or F4& LM, respectively. Caloric intaMe and body Yeight Yere recorded every hs. After YeeMs, mice Yere euthanized and plasma samples Yere collected. Epididymal adipose tissue EAT Yas dissected, Yeighted and processed for q2C4 and cellular analysis. Stromal vascular fraction S8F cells Yere isolated from EAT and processed for gene expression analysis. F4& and F4& LM shoYed an increased in caloric intaMe 2 . vs. CTR and CT4 LM, but only the F4& mice shoYed an increase in Yeight gain and EAT mass 2 . vs. CTR, CTR-Lk and FRD-Lk). Plasmatic triglycerides levels Yere higher in F4& mice 2 . vs CT4 and CT4 LM and revert to control levels in F4& LM group 2 . , Yhile blood glucose did not display any difference betYeen groups. The m40A expression of functional adipose tissue genes 1b, L2L, Adiponectin, )24 and pro inflammatory genes +0FJand TNFα in EAT Yere assessed. All these genes Yere higher in F4& mice, but Yere reverted Yith the lactobacillus administration F4& LM, 2 . . For the S8F from F4& mice, Yhere the immune cells are in high proportion, the expression levels of +0FJ and TNFα Yere higher 2 . vs CT4 and CT4 LM, Yhile for F4& LM the expression values Yere similar to CT4 mice. 9ith this preliminary results Ye can state that the administration of .. Mefiri prevents the deleterious effects of a F4&, impacting the Yeight gain and the functional profile and pro inflammatory state of the EAT.