MODULATORY EFFECT OF TESTOSTERONE ON THE THERMOGENIC ACTIVITY OF BEIGE ADIPOCYTES FROM RETROPERITONEAL ADIPOSE TISSUE

HARNICHAR AE; SPINED E; REY MA; ZUBIRÍA MG; GIOVAMBATTISTA A

Mar del Plata

Congreso; LXI Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC); 2016

It is well known that androgens modulate adipose tissue (AT) distribution and function. We earlier showed the effect of the lack of testosterone (T) on retroperitoneal AT (RPAT) function from orchidectomized rats and the inhibitory action of T on the adipogenic potential of adipocyte precursor cells (APCs) in vitro. We now studied the effect of T, in vivo and in vitro, on the thermogenic activity of beige adipocytes from RPAT. For this aim, RPAT pads were dissected from adult male control (CTR), pre-pubertally orchidectomized (ODX) and pair-fed control (CTR-PF) rats. Pads were processed for histology analysis and UCP-1 quantification (RT-PCR). In addition, APCs from adult male rats were isolated and cultured up to confluence, then cells were induced to differentiate (4 days) with a pro-browning cocktail in the absence or presence of 0.1 µM T (basal (B) or T, respectively); thereafter cells remained in culture medium without or with T. On differentiation day 8, we added 10 µM forskolin (FSK) for 4 hs to a subset of B or T cells: B without FSK (B-B), B with FSK (B-FSK), T without FSK (T-B), T with FSK (T-FSK). Cells were then processed to quantify UCP-1. We found that T decreased UCP-1 expression in in vitro differentiated adipocytes (p