IMBICE   05372
INSTITUTO MULTIDISCIPLINARIO DE BIOLOGIA CELULAR
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Both Type 1 and Type 2 Corticoid Receptors Modulate Glucocorticoids Effects on Adipocyte Precursor Cells
Autor/es:
ZUBIRÍA, MG.; SPINEDI,E.; GIORDANO, A.; GIOVAMBATTISTA, A.; PORTALES, A.
Lugar:
Boston
Reunión:
Congreso; 98th Annual Meeting of the Endocrine Society; 2016
Resumen:
Glucocorticoids are known to be involved in several functions of adipose tissue (AT). We have previously shown that dexamethasone (DXM) impacts on adipocyte precursor cells (APCs) from abdominal AT by enhancing their adipogenic capacity and number, although the role of type 1 (mineralocorticoid receptor, MR) and type 2 (glucocorticoid receptor, GR) corticoids receptors in these processes is still unclear. We have now evaluated whether MR or/and GR activities are critical for these DXM effects. For this aim, stromal vascular fraction cells isolated from retroperitoneal AT (RPAT), aseptically dissected from adult male S-D rats, were cultured until they reached confluence. Then, we added DXM alone (0.25 µM, DXM) or in combination with a GR inhibitor (RU486 1µM, DXM-RU), or an MR inhibitor (Spironolactone 10µM, DXM-SP) or both (DXM-RU-SP) for 48 h (pre-treatment). Culture medium without DXM was used for control cells (CTR). The expression of a specific adipocyte competency marker (PPAR-γ2) and corticoid receptors (MR and GR) was quantified in APCs after pre-treatment (qPCR Real Time). Additionally, differentiation was induced using the conventional cocktail (DIM) and the mRNA levels of PPAR-γ2 and C/EBPα on differentiation day 4 (Dd4) were determined. The percentage of PPAR-γ positive cells was determined by immunofluorescence (IF) on the same day. Finally, intracellular lipid content (Oil-Red O) was measured on Dd 10. Our data showed an increase of PPAR-γ2 gene expression in DXM cells after pre-treatment (P