Adición de DYS438 en haplotipos de cromosoma Y de Amerindios

CATANESI C.I.; SILBESTRO M.; MÉNDEZ M.G.; VIDAL RIOJA L.

La Plata

Congreso; IX Congreso de la Sociedad de Ciencias Morfológicas; 2007

Sociedad de Ciencias Morfológicas de La Plata

(RESUMEN PUBLICADO EN INGLÉS) INTRODUCTION The study of Y-chromosome variation is useful to clarify the origin and geographic procedence of humans. The Y-chromosome is paternally inherited, and a man transmits his Y-specific genetic polymorphisms to all his male descendants, conforming a male lineage. Among the different kinds of Y-chromosome polymorphisms, the microsatellite or STR (short tandem repeat) loci are very informative due to their high allelic variability; DYS438 is a STR of pentanucleotide repeated sequence. MATERIALS AND METHODS We analyzed DNA from 96 Amerindian males, 83 of them belonging to the Gran Chaco ethnic groups Mocoví (n=27), Chorote (n=12), Wichí (n=14), Toba (n=4), Lengua (n=17), and Ayoreo (n=9), and 13 to the Patagonian Tehuelche. DYS438 was analyzed through PCR and  6% denaturing polyacrylamide gel electrophoresis. Gels were stained with AgNO3 for visualization of bands. RESULTS AND DISCUSSION We found alleles 10, 11, 12 and 13, but only two or three of them in every ethnic group, being allele 11 the most frequent for all of the tribes, and allele 13 only found among Tehuelche. Several individuals have been previously analyzed for other Y-STRs which are inherited altogether, composing Y-haplotypes. The inclusion of this marker, allowed to differentiate Tehuelche individuals who previously shared identical haplotypes. However, for the Chaco samples, this marker did not increase haplotype diversity. We conclude, the variability of DYS438 is lower than that of other Y-markers (eg. (DYS385 and DYS392) in Amerindians. The analysis of a higher number of samples will allow in the future to confirm these results.