IMBICE   05372
INSTITUTO MULTIDISCIPLINARIO DE BIOLOGIA CELULAR
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Unveiling the CRF Neurons of the Amygdala: Neuroanatomical and Functional Characterization using a Novel Transgenic Mouse Model
Autor/es:
DE FRANCESCO P N; VALDIVIA S; CABRAL A; PATRONE A; REYNALDO M; PERELLÓ M
Lugar:
Huerta Grande, Córdoba
Reunión:
Congreso; XXIX CONGRESO ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACION EN NEUROCIENCIAS; 2014
Institución organizadora:
Sociedad Argentina de Investigación en Neurociencias
Resumen:
The amygdalar corticotrophin releasing factor (CRF)-producing neurons have been implicated in mediating behavioral and physiological responses related to fear, anxiety, stress and reward. Yet, the study of this set of neurons has been hindered by their challenging identification. By means of a novel transgenic mouse line in which humanized green fluorescent protein (GFP) is under the control of the CRF promoter (CRF-GFP mice), we were able to overcome this issue, allowing for a detailed neuroanatomical insight as well as serving as functional exploratory tool. CRF neurons were observed mainly along the IPAC, with a distribution ranging from the medial part of the CeA to the posterior part of the anterior commissure. Using c-Fos as a marker of neuron activation we explored the response of these neurons by exposing CRF-GFP mice to different experimental paradigms known to activate this region: ghrelin treatment, melanocortin 4 receptor agonist treatment, a conditioned taste aversion paradigm, a high fat diet binging paradigm, a high fat diet withdrawal paradigm, i.p. LPS treatment, a social defeat protocol, and a fasting-refeeding protocol. Of all the cited conditions, only the former three protocols showed a significant increase of c-fos expression in CRF neurons, suggesting they are mainly involved in specific responses related to these paradigms. Overall, this novel CRF-GFP line provides a powerful tool to investigate this intriguing neuronal subset.