Nanoberries: further characterization of liposomal polyphenols and in vitro photoprotection against UV attack

BUCCI, PAULA L; MONTANARI, JORGE A.M.; SILVIA DEL VALLE ALONSO

Sierra de la Ventana

Congreso; SAB 2014 - XLIIi Reunión anual de la Sociedad Argentina de Biofísica; 2014

Sociedad Argentina de Biofísica

Nanoberries1 are ultradeformable liposomes loaded with an ethanolic extract rich in polyphenols from blueberry (Vaccinium myrtillus, variety Millenia). They are potentially useful as delivery systems for photoprotection against UV-mediated skin damage. We prepared the liposomes with soy phosphatidylcholine and sodium cholate as a border activator, at 0.223 extract/lipid w/w. Their phenolic and anthocyanine content, drug to lipid ratio, size and Zeta potential after manual extrusion were determined, and we obtained images by Scanning Electron Microscopy after drying the Nanoberries under different conditions: lyophilization and vacuum dry2. With the aim of assessing their photoprotection capacity against UV attack, we performed experiments on HaCaT cells. A calibration curve of phototoxicity was obtained by viability determination after exposure to different amounts of energy from an UV source. Then, the protection against phototoxicity by Nanoberries and free ethanolic extracts of blueberry were assessed. Acknowledgements: We thank ?The Berry Store? for kindly providing blueberries for this research work, and Dr. Rebeca Oliveira de Souza (Fcfrp, USP, Brazil) for her help with phototoxicity assays.   References 1 Montanari et al. Journal of Cosmetic Sciences. 2013. 469. 2 Montanari et al. International Journal of Pharmaceutics. 2009. 184.