IMBICE   05372
INSTITUTO MULTIDISCIPLINARIO DE BIOLOGIA CELULAR
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Folic acid: pro-oxidant action and characterization of a system based in SPC liposomes
Autor/es:
MARINA MARSANASCO; VALERIA CALABRÓ; BARBARA PIOTRKOWSKI; NADIA CHIARAMONI; SILVIA DEL VALLE ALONSO
Lugar:
Carlos Paz, Cordoba
Reunión:
Congreso; XLII Reunión Anula de la Sociedad Argentina de Biofísica; 2013
Institución organizadora:
Sociedad Argentina de Biofísica
Resumen:
The aim of this work was to study the antioxidant action of folic acid (FA) and characterize liposomes encapsulating it before and after pasteurization. Liposomes were obtained by the Bangham method with soy phosphatidylcholine (SPC) alone and in combination with stearic acid (SA) or calcium stearate (CAS). VC alone or in combination with vitamin E (VE) was added. Oxidative stability was studied with TBA and ORAC methods. Surface charge (Z potential), membrane packing (MC540 and laurdan), size distribution and rheological behavior were studied. Pro-oxidant action of AF was observed with TBA method, even after pasteurization. The VE resists the pro-oxidant effect of the FA. With the ORAC method, no effect was observed with FA alone or in combination with VE. The three systems with FA had similar size distributions and Z potential values. FA favors entry of merocyanine 540 in SPC and SPC:SA and increased laurdan GP when combined with VE in the three formulations . Although the heat treatment decreased the viscosity of SPC and SPC:SA and increased the SPC:CaS, all systems showed Newtonian behavior, which made these systems attractive for industrial applications.