IMBICE   05372
INSTITUTO MULTIDISCIPLINARIO DE BIOLOGIA CELULAR
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Activity change in zebrafish exposed to polymeric liposomes and BSA nanoparticles loaded with drugs
Autor/es:
SIRI, MACARENA; FERNANDEZ RUOCCO, MARÍA JULIETA; MAROTTA CRISTIAN HERNAN; PRIETO, JIMENA; CHIARAMONI, NADIA; ALONSO, SILVIA DEL VALLE
Lugar:
San Javier, Tucuman
Reunión:
Congreso; XLI Reunión Anual de la Sociedad de Biofísica; 2012
Institución organizadora:
Sociedad Argentina de Biofisica
Resumen:
Zebrafish, is an exciting new model to work in vivo due to the advantages ir presents: easy to work with, low cost maintenance, and high progeny at once. The embryonic development is well described and is easy to control the condition in wich is carried on. BSA nanoparticles(BSAn) are of a diameter of 20 nm. Different characterizations studies described the nano particle as more compact molecule than the protein by it´s own due to the high content of alpha-helix. The encapsulation was better trough not in a considerable way, and the drug release was more stable and controlled. On the other hand, liposomes are considered to be useful as drug delivery systems. One particular type of liposomes is the polymeric ones. They can be obtained by photopolymerization and they are very rigid and stable. In this work, we studied the effect of the BSAn bound to the antitumoral drug Emodin, on the activity of zebrafish larvae. Emodin presents great toxicity. Almost no effect was observed in larvae treated with the complex BSAn:Emodin. These were also compared to larvae exposed to BSA and BSA:Emodin. The BSA showed a great hyperactivity on the larvae, whereas the complex, seemed to cause a toxic effect similar to the one caused by drug alone. We have also observed the effect caused by the polymeric liposomes on the spontaneus swim on the larvae. Results shown no toxic effect on any of the larvae tested.