Lipid-polymer membranes as carrier for L-tryptophan: biophysics and metabolic properties

FERNANDEZ RUOCCO MARIA JULIETA; SIRI MACARENA; MAROTA, HERNAN; PRIETO MARIA JIMENA; ALONSO, SILVIA DEL VALLE; CHIARAMONI, NADIA SILVIA

Tucuman

Congreso; XLI Reunion Anual de la Sociedad Argentina de Biofisica; 2012

Sociedad Argentina de Biofisica

A study of polymeric liposomes encapsulating L-tryptophan was carried out with the main goal of characterizing them as drug delivery systems [1, 2]. Polymeric liposomes were obtained by UV irradiation of vesicles with 1,2-bis(10,12-tricosadiynoyl)-sn-glycero-3-phosphocholine (DC8,9PC) and 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) in 1:1 molar ratio, and also obtained in presence of 10 and 50 mol % of L-tryptophan (respect to total lipid concentration). Polymerization efficiency was studied spectrofotometrically [3]. Also, bilayer packing at the polar head region was followed with the probe Merocyanine 540 (MC540) and interactions between lipopolymer and L-tryptophan were studied by FTIR [4, 5]. Results showed that high L-tryptophan concentration induced a higher amount of polymer units due to the L-tryptophan better accommodation of the polar head region. Stability of lipopolymers with different amounts of L-tryptophan was studied through release profiles. Polymeric liposomes were able to retain around 80 % of the L-tryptophan after 24 hour. Metabolic activity of the Caco-2 cell line was also studied in the presence of polymeric liposomes and L-tryptophan. Cytotoxic effects were low after aminoacid addition [6]. In summary, lipopolymers are feasible to be applied in human health. References:. 1. Temprana, C.F; Duarte, E.L., et al. Langmuir, 2010. 26: 10084-92. 2. Wurtman, R.J. and J.J. Wurtman, The Journal of Nutritional Biochemistry, 1998. 9: 511-515. 3. Chiaramoni N.S., Speroni, L., et al. Biotechnol. Lett, 2007. 29: 1637-1644. 4. Chiaramoni N.S., Baccarini, L.C., et al. J. Biol. Phys, 2008. 34: 179-188 5. Popova, A.V. and D.K. Hincha, Chem Phys Lipids, 2004. 132: 171-184 6. Chiaramoni N.S., Gasparri, J., et al. J. Liposomes Res, 2010. 3: 191-201. Acknowledgements: CONICET, MINCyT, UNQ.