IMBICE   05372
INSTITUTO MULTIDISCIPLINARIO DE BIOLOGIA CELULAR
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Scanning ion occlusion Spectroscopy: A new tool to study nanoparticles
Autor/es:
LEANDRO J MARTINEZ; SILVIA SOTO ESPINOSA; SILVIA ALONSO; MARIANO GRASSELLI
Lugar:
San Javier
Reunión:
Simposio; XLI Reunión Anual de la Sociedad de Biofísica; 2012
Institución organizadora:
SAB
Resumen:
Our laboratory is interested in developing functional nanoparticles based on polymeric materials for applications in the area of downstream processing. Our aim is to explore potential applications of their properties such as the particle size, charges and charge density. Dynamic Light Scattering is the convention tool of analysis to these materials; however it has some limitation especially in heterogeneous dispersions. In this work we introduce a novel equipment to measure particle size and charge which is based on the analysis of each particle when it path through a single pore. This pore is stretchable and it can be tuned to optimize the pore size to a particular experimental system to distinguish between nanoparticle populations of similar size. Scanning Ion Occlusion Spectroscopy allows particle-by-particle measurement of the size, concentration (particles per mL) and relative surface charge distribution of particles simultaneously determined with high accuracy. A wide range of biological and synthetic particle types, spanning 50 nm to 10 μm, can be measured by this technique. In this work are shown preliminary results of functional nanoparticles based on poly(methylmethacrylate) modified nanosilica by using this Tunable Resistive Pulse Sensing methodology, which showed average sizes between 190 and 260 nm. L.J.M. and S.L.S.E. thanks the ANPCyT for the fellowship. M.G. and S.A. are researchers from CONICET. This work was partially supported by grants from Universidad Nacional de Quilmes, IAEA and MINCyT, Argentina.