DISTORTED IN VITRO ADIPOGENIC CAPACITY OF STROMAL-VASCULAR FRACTION (SVF) CELLS IN A MODEL OF HYPOTHALAMIC OBESITY

ZUBIRIA GUILLERMINA; VIDAL BRAVO, JUANA; GAILLARD, R; SPINEDI, E; GIOVAMBATTISTA, A

Buenos Aires

Congreso; The Second International Congress on Abdominal Obesity; 2011

International Chair on Cardiometabolic Risk

Objective: The aim of the present study was to evaluate the adipogenic capacity of retroperitoneal (RP) fat pad SVF cells in a hyperadipose male rat model, due to neonatal monosodium L-glutamate (MSG) treatment.Methods: Isolated RP SVF cells in culture were allowed to proliferate up to reach confluence. Then preadipocyte differentiation was accordingly induced (day 0), and cells were maintained in culture up to 10 days post-differentiation. Preadipocyte factor-1 (Pref-1) mRNA was quantified by RT-PCR real time on both day 0 and day 2 post-differentiation. Additionally, between days 0 and 10 of the differentiation period, media leptin (LEP) concentrations were monitored. Intracellular lipid content (Oil-Red O) and gene expression (LEP and PPARg mRNA levels) were also examined. Results: MSG rats were hyperleptinemic, and their SVF cells revealed a high (p< 0.05 vs. CTR) Pref-1 mRNA expression on day 0 and on day 2 post-differentiation. Between days 6 and 10 post-differentiation, MSG cells showed diminished (p< 0.05 vs. CTR) LEP release into the medium. Moreover, between days 4 and 10 post-differentiation, cell lipid content and mRNA levels of PPARg and LEP were lower (p< 0.05 vs. CTR) in differentiating MSG cells. Conclusions: Our study suggests that in the adult male MSG rat, the in vitro adipogenic capacity seems to be delayed. Therefore, it is possible to speculate that the leptin-enriched endogenous environment characterizing MSG rats could be partially responsible for distorted RP fat pad SVF cells adipogenesis.