Modulation of TGF-Beta1 and VEGF-A expression in the testes of transgenic over-expressing hCG

GONZáLEZ C, RULLI S, GONZáLEZ B, HUHTANIEMI I, CALANDRA RS, GONZáLEZ-CALVAR SI

Belo Horizonte, Brasil

Simposio; 1st International Symposium on Animal Biology of Reproduction; 2006

Federal University of Minas Gerais, Belo Horizonte, Brazil

��High and chronic levels of hCG induce testicular TGF-â1 expression in TG mice at 8 weeks of age.High and chronic levels of hCG induce testicular TGF-â1 expression in TG mice at 8 weeks of age. ��There was an increase in the expression of endoglin in TG mice at 3 weeks of age, suggesting its involvement in testicular cell proliferation.There was an increase in the expression of endoglin in TG mice at 3 weeks of age, suggesting its involvement in testicular cell proliferation. ��The expression of VEGFA and its receptor VEGF-R2 is higher in TG mice at both ages compared to WT. The presence of VEGFA120 and VEGFA164 isoforms was observed in TG mice at both ages, while VEGFA188 was only detected in TG mice at 3 weeks.The expression of VEGFA and its receptor VEGF-R2 is higher in TG mice at both ages compared to WT. The presence of VEGFA120 and VEGFA164 isoforms was observed in TG mice at both ages, while VEGFA188 was only detected in TG mice at 3 weeks. ��TGF-â1 might regulate the expression of VEGFA in a dose-dependent fashion in TG mice at 8 weeks of age. The biphasic effect observed prompts us to speculate that low concentrations of TGF-â1 enhanced the expression of VEGFA and endoglin, probably leading to cell hypertrophy and hyperplasia, while higher concentrations blocked this effect.TGF-â1 might regulate the expression of VEGFA in a dose-dependent fashion in TG mice at 8 weeks of age. The biphasic effect observed prompts us to speculate that low concentrations of TGF-â1 enhanced the expression of VEGFA and endoglin, probably leading to cell hypertrophy and hyperplasia, while higher concentrations blocked this effect.â1 enhanced the expression of VEGFA and endoglin, probably leading to cell hypertrophy and hyperplasia, while higher concentrations blocked this effect. ��Immunohistochemistry show the presence of VEGFA in mouse Leydig cells, which suggest a key role for this factor.Immunohistochemistry show the presence of VEGFA in mouse Leydig cells, which suggest a key role for this factor. ��The temporal expression of TGF-â1, endoglin and VEGFA, might modulate the differentiation and development of the testes.The temporal expression of TGF-â1, endoglin and VEGFA, might modulate the differentiation and development of the testes.