IMBICE   05372
INSTITUTO MULTIDISCIPLINARIO DE BIOLOGIA CELULAR
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Fructose-Induced Metabolic Syndrome: its impact upon the Adipogenic Process.
Autor/es:
ZUBIRIA G; VIDAL BRAVO J; FARIÑA JP; GAGLIARDINO JJ; SPINEDI E; GIOVAMBATTISTA A
Lugar:
Orlando FL
Reunión:
Congreso; 2011 NAASO Anual Meeting; 2011
Institución organizadora:
NAASO
Resumen:
Background: Obesity represents a major worldwide health problem and it is characterized by an increased mass and cell size of adipose tissue (AT) and a distorted pattern of adipokine secretion. Nowadays, the increase in fructose intake appears as one of the main causes for the development of metabolic syndrome, obesity and diabetes. We have previously shown that administration of a fructose rich diet (FRD)(10 % w/v in the drinking water) to normal adult male rats for 3 weeks induced an increase in the mass of abdominal AT and adipocyte dysfunction, probably secondary to an enhanced oxidative stress. Aim: to evaluate the impact of the FRD on in vitro adipogenesis, a process encompassing pre-adipocyte differentiation into mature AT cells. Procedures: stromal vascular fraction (SVF) cells were isolated from retroperitoneal AT pads of normal (control, CT) and FRD adult male rats. Before starting differentiation, cultured cells were daily counted up to day 9th (pre-differentiation period, PDP). Thereafter, cells differentiation was induced in a 10 day-schedule. Between days 0 and 10 of the differentiation period (DP), culture media were removed (every 48 h), kept frozen (-20 C) for leptin (LEP) concentration measurement and replaced by fresh media. At the end of the DP, cell lipid content (Oil-Red O) and gene expression [LEP, adiponectin (ADIPOQ), PPARg and IRS-1 mRNAs, by real time PCR] were monitored. Results: Although during the PDP the proliferative capacity of SVF cells was similar in both groups (CT and FRD), at the end of the DP, FRD cells displayed a significantly higher lipid content and LEP release (p< 0.05 vs. CT). These cells also have significantly higher concentration of LEP, ADIPOQ and IRS-1 mRNA (p < 0.05 vs. CT), whereas that of PPARg was only slightly increased. Conclusions: Our data demonstrate that administration of a FRD for a short time-period increases the cell capacity to store lipids, probably due to an enhancement of the insulin signaling system of mature adipocytes. Accordingly, the intake of this FRD could accelerate the adipogenic process contributing in that way to the enlargement of the AT mass and its dysfunction. These altered processes would lead to the development of the metabolic syndrome (Supported by PICT 2007-1051).