Growth hormone secretagogue receptor signaling affects hight-fat intake independently of plasma levels of ghrelin and leap2, in a 4-day binge eating model

CORNEJO, MARÍA PAULA; REYNALDO, MIRTA; PERELLO, MARIO; CASTROGIOVANNI, DANIEL; MARIE, JACKY; SCHIÖTH, HELGI B.; FEHRENTZ, JEAN-ALAIN

NEUROSCIENCE

PERGAMON-ELSEVIER SCIENCE LTD

Lugar: Amsterdam; Año: 2019 vol. 31

0306-4522

Ghrelin is a stomach-derived hormone that regulates a variety of biological functions such as food intake, gastrointestinalfunction and blood glucose metabolism, among others. Ghrelin acts via the growth hormone secretagogue receptor (GHSR), aG-protein-coupled receptor located in key brain areas that mediate specific actions of the hormone. GHSR is highly expressedin the nucleus of the solitary tract (NTS), which is located in the medulla oblongata and controls essential functions, includingorofacial, autonomic, neuroendocrine and behavioral responses. Here, we used a mouse model, in which the expression ofenhanced green fluorescent protein (eGFP) is controlled by the promoter of GHSR (GHSR-eGFP mice), to gain neuroanatomicaland functional insights of the GHSR-expressing neurons of the NTS. We found that GHSR-expressing neurons ofthe NTS are segregated in clusters that were symmetrically distributed to the midline: (1) a pair of rostral clusters, and (2)a caudal and medially located cluster. We also identified that a subset of GHSR neurons of the caudal NTS are GABAergic.Finally, we found that rostral NTS GHSR neurons increase the levels of the marker of neuronal activation c-Fos in miceexposed to fasting/refeeding or high-fat diet bingeing protocols, while caudal NTS GHSR neurons increase the levels ofc-Fos in mice exposed to gastric distension or LiCl-induced malaise protocols. Thus, current data provide evidence thatghrelin receptor signaling seems to target segregated clusters of neurons within the NTS that, in turn, may be activated bydifferent stimuli.