IMBICE   05372
INSTITUTO MULTIDISCIPLINARIO DE BIOLOGIA CELULAR
Unidad Ejecutora - UE
artículos
Título:
A simple in silico strategy identifies candidate biomarkers for the diagnosis of liver fibrosis in morbidly obese subjects
Autor/es:
GIRAUDI, PABLO J.; CHACKELEVICIUS, CARLA M.; MACOR, DANIELE; SOARDO, GIORGIO; TIRIBELLI, CLAUDIO; GAMBARO, SABRINA E.; GIURICIN, MICHELA; CROC?, LORY S.; DE MANZINI, NICOL?; PALMISANO, SILVIA; ORNELAS ARROYO, SOFIA; SILVESTRI, MARTA; BONAZZA, DEBORAH; ZANCONATI, FABRIZIO; ROSSO, NATALIA
Revista:
LIVER INTERNATIONAL
Editorial:
WILEY-BLACKWELL PUBLISHING, INC
Referencias:
Año: 2017
ISSN:
1478-3223
Resumen:
Background and aimsNon-alcoholic fatty liver disease (NAFLD) is a chronic liver disorder, tightly associated with obesity.The histological spectrum of the disease ranges from simple steatosis to steatohepatitis, with different stages of fibrosis, and fibrosis stage is the most significant predictor of mortality in NAFLD. Liver biopsy continues to be the gold standard for its diagnosis and reliable non-invasive diagnostic tools are unavailable. We investigated the accuracy of candidate proteins, identified by an in silico approach, as biomarkers for diagnosis of fibrosis.Methods71 morbidly obese (MO) subjects with biopsy-proven NAFLD were enrolled, and the cohort was subdivided according to minimal (F0/F1) or moderate (F2/F3) fibrosis. The plasmatic level of CD44 antigen (CD44), secreted protein acidic and rich in cysteine (SPARC), epidermal growth factor receptor (EGFR) and insulin-like growth factor 2 (IGF2) were determined by ELISA. Significant associations between plasmatic levels and histological fibrosis were determined by correlation analysis and the diagnostic accuracy by the area under receiver operating characteristic curves (AUROC).Results82% of the subjects had F0/F1 and 18% with F2/F3 fibrosis. Plasmatic levels of IGF2, EGFR and their ratio (EGFR/IGF2) were associated with liver fibrosis, correlating inversely for IGF2 (p