IMBICE   05372
INSTITUTO MULTIDISCIPLINARIO DE BIOLOGIA CELULAR
Unidad Ejecutora - UE
artículos
Título:
Association with Amino Acids Does Not Enhance Efficacy of Polymerized Liposomes As a System for Lung Gene Delivery
Autor/es:
BANDEIRA, ELGA; LOPES-PACHECO, MIQUEIAS; CHIARAMONI, NADIA SILVIA; FERREIRA, DEBORA; FERNANDEZ RUOCCO MARIA JULIETA; PRIETO, MARIA JIMENA; MARON-GUTIERREZ, TATIANA; ROCCO, PATRICIA R.M.; ALONSO, SILVIA DEL VALLE; MORALES, MARCELO
Revista:
Frontiers in Physiology
Editorial:
Frontiers
Referencias:
Lugar: Ohio; Año: 2016 vol. 7
Resumen:
Developmentofimproveddrugandgenedeliverysystemsdirectlyintothelungsishighlydesirablegiventheimportantburdenofrespiratorydiseases.Weaimedtoevaluatethesafetyandefficacyofliposomescomposedofphotopolymerizedlipids[1,2-bis-(tricosa-10,12-diynoyl)-sn-glycero-3-phosphocholine]associatedwithaminoacidsasvectorsforgenedeliveryintothelungsofhealthyanimals.Lipopolymervesicles,inparticular,aremorestablethanothertypesofliposomes.Inthisstudy,lipopolymerswereassociatedwithL-arginine,L-tryptophan,orL-cysteine.Wehypothesizedthattheadditionoftheseaminoacidswouldenhancetheefficacyofgenedeliverytothelungsbythelipopolymers.L-Arginineshowedthehighestassociationefficiencyduetoitspositivechargeandbettersurfaceinteractions.Noneoftheformulationscausedinflammationoralteredlungmechanics,suggestingthattheselipopolymerscanbesafelyadministeredasaerosols.AllformulationswereabletoinduceeGFPmRNAexpressioninlungtissue,buttheadditionofaminoacidsreduceddeliveryefficacywhencomparedwiththesimplelipopolymerparticle.Theseresultsindicatethatthissystemcouldbefurtherexploredforgeneordrugdeliverytargetinglungdiseases.