IMBICE   05372
INSTITUTO MULTIDISCIPLINARIO DE BIOLOGIA CELULAR
Unidad Ejecutora - UE
artículos
Título:
Cytocompatibility, cytotoxicity and genotoxicity analyses of dental
Autor/es:
REIGOSA M.1 -2 LABARTA V.1 MOLINARI G. AND BERNALES D.
Revista:
JBAG
Editorial:
Sociedad Argentina de Genética
Referencias:
Lugar: Buenos Aires; Año: 2008 vol. 19 p. 43 - 48
Resumen:
Different materials are used for prostheses in dental medicine, though titanium is the
most frequent. In the present report, several commercial dental implant and their
potential capacity to integrate to bone tissues have been analysed by means of
cytotoxicity, genotoxicity and cytocompatibility techniques. Cultures from
osteosarcoma-derived UMR 106 cell line were used for bioassays since they showed
several osteoblast properties. Samples of dental implants provided by B&M national
company (ASTM titanium 8348 GR2, acid-etched surface) were compared with some
others commercialized by foreign firms in Argentina (Nobel: anodic-oxidized surface,
and 3i: sandblasted surface). Using the neutral red uptake test, lysosome and
alkaline phosphatase enzymatic activities were measured in order to assess
cytotoxicity level. Acridine ethidium/orange bromide technique was used to determine
cell viability, and genotoxicity was analysed using the comet assay. Results from
bioassays showed high biocompatibility and no significant differences among dental
samples evaluated. Techniques employed for the present study proved to be a useful
tool for the analysis of other materials used in dental medicine.