IMBICE   05372
INSTITUTO MULTIDISCIPLINARIO DE BIOLOGIA CELULAR
Unidad Ejecutora - UE
artículos
Título:
Characterization of gastric and neuronal histaminergic populations using a transgenic mouse model
Autor/es:
WALKER AK; PARK WM; CHUANG J-C; PERELLO M; SAKATA I; OSBORNE-LAWRENCE S; ZIGMAN JM
Revista:
PLOS ONE
Editorial:
PUBLIC LIBRARY SCIENCE
Referencias:
Lugar: San Francisco; Año: 2013 p. 60276 - 60277
ISSN:
1932-6203
Resumen:
Histamine is a potent biogenic amine that mediates numerous
physiological processes throughout the body, including digestion, sleep,
and immunity. It is synthesized by gastric enterochromaffin-like cells,
a specific set of hypothalamic neurons, as well as a subset of white
blood cells, including mast cells. Much remains to be learned about
these varied histamine-producing cell populations. Here, we report the
validation of a transgenic mouse line in which Cre recombinase
expression has been targeted to cells expressing histidine decarboxylase
(HDC), which catalyzes the rate-limiting step in the synthesis of
histamine. This was achieved by crossing the HDC-Cre mouse line with
Rosa26-tdTomato reporter mice, thus resulting in the expression of the
fluorescent Tomato (Tmt) signal in cells containing Cre recombinase
activity. As expected, the Tmt signal co-localized with
HDC-immunoreactivity within the gastric mucosa and gastric submucosa and
also within the tuberomamillary nucleus of the brain. HDC expression
within Tmt-positive gastric cells was further confirmed by quantitative
PCR analysis of mRNA isolated from highly purified populations of
Tmt-positive cells obtained by fluorescent activated cell sorting
(FACS). HDC expression within these FACS-separated cells was found to
coincide with other markers of both ECL cells and mast cells. Gastrin
expression was co-localized with HDC expression in a subset of
histaminergic gastric mucosal cells. We suggest that these transgenic
mice will facilitate future studies aimed at investigating the function
of histamine-producing cells.