IMBICE   05372
INSTITUTO MULTIDISCIPLINARIO DE BIOLOGIA CELULAR
Unidad Ejecutora - UE
artículos
Título:
Mitochondrial Heteroplasmy in Control Region of South American camelids
Autor/es:
MATÉ M.L, DI ROCCO F, ZAMBELLI A,VIDAL-RIOJA L.
Revista:
JOURNAL OF SMALL RUMINANT RESEARCH
Editorial:
Elsevier
Referencias:
Año: 2007 vol. 71 p. 123 - 129
ISSN:
0921-4488
Resumen:
Abstract
In the presentwork, polymerase chain reaction-single strand conformation polymorphism and sequencing were used to investigate
the length and nucleotide variability in the Control Region mitochondrial DNA of the four South American camelid species from
Argentina.To asses these the complete Control Region of 20 animals, 5 each of guanaco, llama, alpaca and vicuna species were cloned.
Seventy-three clones corresponding to the 20 animals were screened and 7 different SSCP patterns were identified. Sequencing
of all clones showed 9 different haplotypes contained in the 350 bp hypervariable segment of the Control Region. Interestingly,
3 guanacos, 3 vicunas, 3 alpacas and 1 llama were heteroplasmic for different nucleotide positions. The screening of the Control
Region mitochondrial DNA in blood samples from about 200 wild guanacos from Argentine Patagonia supported the above results.
After comparison with other vertebrate species, we concluded that nucleotide substitutions are the main cause of heteroplasmy
found in Control Region mitochondrial DNA of these taxa.