IMBICE   05372
INSTITUTO MULTIDISCIPLINARIO DE BIOLOGIA CELULAR
Unidad Ejecutora - UE
artículos
Título:
2,4-Dichlorophenoxyacetic acid (2,4-D)-induced (a herbicide) cytogenetic
Autor/es:
SONIA SOLONESKI, NORMA V. GONZA´LEZ, MIGUEL A. REIGOSA, MARCELO L. LARRAMENDY*
Revista:
CELL BIOLOGY INTERNATIONAL
Editorial:
ELSEVIER
Referencias:
Año: 2007 p. 1316 - 1322
ISSN:
1065-6995
Resumen:
The genotoxic effects of 2,4-D and its commercial derivative 2,4-D DMA were studied by measuring sister chromatid exchange (SCE) cellcycle progression and mitotic index in human whole blood (WBC) and plasma leukocyte cultures (PLC). Concentrations of 10, 25, 50 and 100 mg herbicide/ml were used during 72 h. In WBC, a significant increase in SCE frequency was observed within the 10e50 mg 2,4-D/ml and 25e100 mg 2,4-D DMA/ml dose range. Contrarily, in PLC, none of the concentrations employed affected the SCEs frequency. A significant delay in cell proliferation was observed in WBC after treatments with 25 and 50 mg 2,4-D/ml and 50 and 100 mg 2,4-D DMA/ml. In PLC, only 100.0 mg 2,4-D/ml altered cell-cycle progression. For both chemicals, a progressive dose-related inhibition of mitotic activity was observed. The results demonstrated that the presence of erythrocytes in the culture system modulated the DNA and cellular damage inflicted by 2,4-D and 2,4- D DMA into human lymphocytes in vitro as well as both 2,4-D and 2,4-D DMAwere more potent genotoxic agents in the presence of human red cells. cells. results demonstrated that the presence of erythrocytes in the culture system modulated the DNA and cellular damage inflicted by 2,4-D and 2,4- D DMA into human lymphocytes in vitro as well as both 2,4-D and 2,4-D DMAwere more potent genotoxic agents in the presence of human red cells. cells. 100.0 mg 2,4-D/ml altered cell-cycle progression. For both chemicals, a progressive dose-related inhibition of mitotic activity was observed. The results demonstrated that the presence of erythrocytes in the culture system modulated the DNA and cellular damage inflicted by 2,4-D and 2,4- D DMA into human lymphocytes in vitro as well as both 2,4-D and 2,4-D DMAwere more potent genotoxic agents in the presence of human red cells. cells. results demonstrated that the presence of erythrocytes in the culture system modulated the DNA and cellular damage inflicted by 2,4-D and 2,4- D DMA into human lymphocytes in vitro as well as both 2,4-D and 2,4-D DMAwere more potent genotoxic agents in the presence of human red cells. cells. delay in cell proliferation was observed in WBC after treatments with 25 and 50 mg 2,4-D/ml and 50 and 100 mg 2,4-D DMA/ml. In PLC, only 100.0 mg 2,4-D/ml altered cell-cycle progression. For both chemicals, a progressive dose-related inhibition of mitotic activity was observed. The results demonstrated that the presence of erythrocytes in the culture system modulated the DNA and cellular damage inflicted by 2,4-D and 2,4- D DMA into human lymphocytes in vitro as well as both 2,4-D and 2,4-D DMAwere more potent genotoxic agents in the presence of human red cells. cells. results demonstrated that the presence of erythrocytes in the culture system modulated the DNA and cellular damage inflicted by 2,4-D and 2,4- D DMA into human lymphocytes in vitro as well as both 2,4-D and 2,4-D DMAwere more potent genotoxic agents in the presence of human red cells. cells. 100.0 mg 2,4-D/ml altered cell-cycle progression. For both chemicals, a progressive dose-related inhibition of mitotic activity was observed. The results demonstrated that the presence of erythrocytes in the culture system modulated the DNA and cellular damage inflicted by 2,4-D and 2,4- D DMA into human lymphocytes in vitro as well as both 2,4-D and 2,4-D DMAwere more potent genotoxic agents in the presence of human red cells. cells. results demonstrated that the presence of erythrocytes in the culture system modulated the DNA and cellular damage inflicted by 2,4-D and 2,4- D DMA into human lymphocytes in vitro as well as both 2,4-D and 2,4-D DMAwere more potent genotoxic agents in the presence of human red cells. cells. and 25e100 mg 2,4-D DMA/ml dose range. Contrarily, in PLC, none of the concentrations employed affected the SCEs frequency. A significant delay in cell proliferation was observed in WBC after treatments with 25 and 50 mg 2,4-D/ml and 50 and 100 mg 2,4-D DMA/ml. In PLC, only 100.0 mg 2,4-D/ml altered cell-cycle progression. For both chemicals, a progressive dose-related inhibition of mitotic activity was observed. The results demonstrated that the presence of erythrocytes in the culture system modulated the DNA and cellular damage inflicted by 2,4-D and 2,4- D DMA into human lymphocytes in vitro as well as both 2,4-D and 2,4-D DMAwere more potent genotoxic agents in the presence of human red cells. cells. results demonstrated that the presence of erythrocytes in the culture system modulated the DNA and cellular damage inflicted by 2,4-D and 2,4- D DMA into human lymphocytes in vitro as well as both 2,4-D and 2,4-D DMAwere more potent genotoxic agents in the presence of human red cells. cells. 100.0 mg 2,4-D/ml altered cell-cycle progression. For both chemicals, a progressive dose-related inhibition of mitotic activity was observed. The results demonstrated that the presence of erythrocytes in the culture system modulated the DNA and cellular damage inflicted by 2,4-D and 2,4- D DMA into human lymphocytes in vitro as well as both 2,4-D and 2,4-D DMAwere more potent genotoxic agents in the presence of human red cells. cells. results demonstrated that the presence of erythrocytes in the culture system modulated the DNA and cellular damage inflicted by 2,4-D and 2,4- D DMA into human lymphocytes in vitro as well as both 2,4-D and 2,4-D DMAwere more potent genotoxic agents in the presence of human red cells. cells. delay in cell proliferation was observed in WBC after treatments with 25 and 50 mg 2,4-D/ml and 50 and 100 mg 2,4-D DMA/ml. In PLC, only 100.0 mg 2,4-D/ml altered cell-cycle progression. For both chemicals, a progressive dose-related inhibition of mitotic activity was observed. The results demonstrated that the presence of erythrocytes in the culture system modulated the DNA and cellular damage inflicted by 2,4-D and 2,4- D DMA into human lymphocytes in vitro as well as both 2,4-D and 2,4-D DMAwere more potent genotoxic agents in the presence of human red cells. cells. results demonstrated that the presence of erythrocytes in the culture system modulated the DNA and cellular damage inflicted by 2,4-D and 2,4- D DMA into human lymphocytes in vitro as well as both 2,4-D and 2,4-D DMAwere more potent genotoxic agents in the presence of human red cells. cells. 100.0 mg 2,4-D/ml altered cell-cycle progression. For both chemicals, a progressive dose-related inhibition of mitotic activity was observed. The results demonstrated that the presence of erythrocytes in the culture system modulated the DNA and cellular damage inflicted by 2,4-D and 2,4- D DMA into human lymphocytes in vitro as well as both 2,4-D and 2,4-D DMAwere more potent genotoxic agents in the presence of human red cells. cells. results demonstrated that the presence of erythrocytes in the culture system modulated the DNA and cellular damage inflicted by 2,4-D and 2,4- D DMA into human lymphocytes in vitro as well as both 2,4-D and 2,4-D DMAwere more potent genotoxic agents in the presence of human red cells. cells. mg herbicide/ml were used during 72 h. In WBC, a significant increase in SCE frequency was observed within the 10e50 mg 2,4-D/ml and 25e100 mg 2,4-D DMA/ml dose range. Contrarily, in PLC, none of the concentrations employed affected the SCEs frequency. A significant delay in cell proliferation was observed in WBC after treatments with 25 and 50 mg 2,4-D/ml and 50 and 100 mg 2,4-D DMA/ml. In PLC, only 100.0 mg 2,4-D/ml altered cell-cycle progression. For both chemicals, a progressive dose-related inhibition of mitotic activity was observed. The results demonstrated that the presence of erythrocytes in the culture system modulated the DNA and cellular damage inflicted by 2,4-D and 2,4- D DMA into human lymphocytes in vitro as well as both 2,4-D and 2,4-D DMAwere more potent genotoxic agents in the presence of human red cells. cells. results demonstrated that the presence of erythrocytes in the culture system modulated the DNA and cellular damage inflicted by 2,4-D and 2,4- D DMA into human lymphocytes in vitro as well as both 2,4-D and 2,4-D DMAwere more potent genotoxic agents in the presence of human red cells. cells. 100.0 mg 2,4-D/ml altered cell-cycle progression. For both chemicals, a progressive dose-related inhibition of mitotic activity was observed. The results demonstrated that the presence of erythrocytes in the culture system modulated the DNA and cellular damage inflicted by 2,4-D and 2,4- D DMA into human lymphocytes in vitro as well as both 2,4-D and 2,4-D DMAwere more potent genotoxic agents in the presence of human red cells. cells. results demonstrated that the presence of erythrocytes in the culture system modulated the DNA and cellular damage inflicted by 2,4-D and 2,4- D DMA into human lymphocytes in vitro as well as both 2,4-D and 2,4-D DMAwere more potent genotoxic agents in the presence of human red cells. cells. delay in cell proliferation was observed in WBC after treatments with 25 and 50 mg 2,4-D/ml and 50 and 100 mg 2,4-D DMA/ml. In PLC, only 100.0 mg 2,4-D/ml altered cell-cycle progression. For both chemicals, a progressive dose-related inhibition of mitotic activity was observed. The results demonstrated that the presence of erythrocytes in the culture system modulated the DNA and cellular damage inflicted by 2,4-D and 2,4- D DMA into human lymphocytes in vitro as well as both 2,4-D and 2,4-D DMAwere more potent genotoxic agents in the presence of human red cells. cells. results demonstrated that the presence of erythrocytes in the culture system modulated the DNA and cellular damage inflicted by 2,4-D and 2,4- D DMA into human lymphocytes in vitro as well as both 2,4-D and 2,4-D DMAwere more potent genotoxic agents in the presence of human red cells. cells. 100.0 mg 2,4-D/ml altered cell-cycle progression. For both chemicals, a progressive dose-related inhibition of mitotic activity was observed. The results demonstrated that the presence of erythrocytes in the culture system modulated the DNA and cellular damage inflicted by 2,4-D and 2,4- D DMA into human lymphocytes in vitro as well as both 2,4-D and 2,4-D DMAwere more potent genotoxic agents in the presence of human red cells. cells. results demonstrated that the presence of erythrocytes in the culture system modulated the DNA and cellular damage inflicted by 2,4-D and 2,4- D DMA into human lymphocytes in vitro as well as both 2,4-D and 2,4-D DMAwere more potent genotoxic agents in the presence of human red cells. cells. e100 mg 2,4-D DMA/ml dose range. Contrarily, in PLC, none of the concentrations employed affected the SCEs frequency. A significant delay in cell proliferation was observed in WBC after treatments with 25 and 50 mg 2,4-D/ml and 50 and 100 mg 2,4-D DMA/ml. In PLC, only 100.0 mg 2,4-D/ml altered cell-cycle progression. For both chemicals, a progressive dose-related inhibition of mitotic activity was observed. The results demonstrated that the presence of erythrocytes in the culture system modulated the DNA and cellular damage inflicted by 2,4-D and 2,4- D DMA into human lymphocytes in vitro as well as both 2,4-D and 2,4-D DMAwere more potent genotoxic agents in the presence of human red cells. cells. results demonstrated that the presence of erythrocytes in the culture system modulated the DNA and cellular damage inflicted by 2,4-D and 2,4- D DMA into human lymphocytes in vitro as well as both 2,4-D and 2,4-D DMAwere more potent genotoxic agents in the presence of human red cells. cells. 100.0 mg 2,4-D/ml altered cell-cycle progression. For both chemicals, a progressive dose-related inhibition of mitotic activity was observed. The results demonstrated that the presence of erythrocytes in the culture system modulated the DNA and cellular damage inflicted by 2,4-D and 2,4- D DMA into human lymphocytes in vitro as well as both 2,4-D and 2,4-D DMAwere more potent genotoxic agents in the presence of human red cells. cells. results demonstrated that the presence of erythrocytes in the culture system modulated the DNA and cellular damage inflicted by 2,4-D and 2,4- D DMA into human lymphocytes in vitro as well as both 2,4-D and 2,4-D DMAwere more potent genotoxic agents in the presence of human red cells. cells. mg 2,4-D/ml and 50 and 100 mg 2,4-D DMA/ml. In PLC, only 100.0 mg 2,4-D/ml altered cell-cycle progression. For both chemicals, a progressive dose-related inhibition of mitotic activity was observed. The results demonstrated that the presence of erythrocytes in the culture system modulated the DNA and cellular damage inflicted by 2,4-D and 2,4- D DMA into human lymphocytes in vitro as well as both 2,4-D and 2,4-D DMAwere more potent genotoxic agents in the presence of human red cells. cells. results demonstrated that the presence of erythrocytes in the culture system modulated the DNA and cellular damage inflicted by 2,4-D and 2,4- D DMA into human lymphocytes in vitro as well as both 2,4-D and 2,4-D DMAwere more potent genotoxic agents in the presence of human red cells. cells. mg 2,4-D/ml altered cell-cycle progression. For both chemicals, a progressive dose-related inhibition of mitotic activity was observed. The results demonstrated that the presence of erythrocytes in the culture system modulated the DNA and cellular damage inflicted by 2,4-D and 2,4- D DMA into human lymphocytes in vitro as well as both 2,4-D and 2,4-D DMAwere more potent genotoxic agents in the presence of human red cells. cells. in vitro as well as both 2,4-D and 2,4-D DMAwere more potent genotoxic agents in the presence of human red cells.