Detection of Streptococcusmutans Genomic DNA in Human DNA Samples Extracted from Saliva and Blood

VIEIRA AR; DEELEY KB; CALLAHAN NF; NOEL JB; ANTOMSHOAA I; CARRICATO WM; SCHULHOF LP; DENENSI RS; GANDHI P; RESICK JM; BRANDON CA; ROZHON C; PATIR A; YILDIRIM M; POLETTA FA; JORGE SANTIAGO LOPEZ CAMELO; CASTILLA EE; ORIOLI I; SEYMEN F; WEYANT RJ; CROUT R; MCNEIL DW; MODESTO A; MARAZITA ML

INTERNATIONAL DENTAL JOURNAL - (Online)

F D I WORLD DENTAL PRESS LTD

Lugar: New York; Año: 2011 vol. 2011 p. 1 - 6

0020-6539

Detection of Streptococcus mutans Genomic DNA in Human DNA Samples Extracted from Saliva and Blood. Vieira AR, Deeley KB, Callahan NF, Noel JB, Anjomshoaa I, Carricato WM, Schulhof LP, Desensi RS, Gandhi P, Resick JM, Brandon CA, Rozhon C, Patir A, Yildirim M, Poletta FA, Mereb JC, Letra A, Menezes R, Wendell S, Lopez-Camelo JS, Castilla EE, Orioli IM, Seymen F, Weyant RJ, Crout R, McNeil DW, Modesto A, Marazita ML. Source Department of Oral Biology, School of Dental Medicine, University of Pittsburgh, Pittsburgh, PA, USA. Abstract Caries is a multifactorial disease, and studies aiming to unravel the factors modulating its etiology must consider all known predisposing factors. One major factor is bacterial colonization, and Streptococcus mutans is the main microorganism associated with the initiation of the disease. In our studies, we have access to DNA samples extracted from human saliva and blood. In this report, we tested a real-time PCR assay developed to detect copies of genomic DNA from Streptococcus mutans in 1,424 DNA samples from humans. Our results suggest that we can determine the presence of genomic DNA copies of Streptococcus mutans in both DNA samples from caries-free and caries-affected individuals. However, we were not able to detect the presence of genomic DNA copies of Streptococcus mutans in any DNA samples extracted from peripheral blood, which suggests the assay may not be sensitive enough for this goal. Values of the threshold cycle of the real-time PCR reaction correlate with higher levels of caries experience in children, but this correlation could not be detected for adults.