Modulation of gamma delta T lymphocyte functionality by glioblastoma cell line U251

SHIROMIZU, CAROLINA MAIUMI; SALAMONE, GABRIELA V.; ROSATO, MICAELA; KEITELMAN, IRENE A.; ROSSO, DAVID A.; CORONEL, JUAN V.; JANCIC, CAROLINA C.

CABA

Congreso; Reunión anual de Sociedades de Biociencias 2020; 2020

SAIC- SAI- SAFIS

Glioblastoma multiforme (GBM) is the most frequent and malignant cause of primary brain tumors in adults; it has poor prognostic, and median survival after diagnostic is less than a year which makes it a top priority for public health. γδ T cells are a subtype of T lymphocyte that induce apoptosis on stressed and malignant cells by sensing their increased expression of phosphorylated molecules. Their ability to expand after stimulation and exert cytotoxicity over tumoral cells is being used in immunotherapy approaches against cancer. In this work we aimed to evaluate the modulation of γδ T cell functionality by human GBM cell line U251 and how it could impact in their antitumoral response. For that purpose, γδ T cells were purified from human peripheral blood mononuclear cell, by using an anti- TCR γδ MicroBead isolation kit. After purification, γδ T cells wereco-cultured with U251 cell line or it?s conditioned medium during 24 hours in the absence or presence of phosphoantigen (E)-4-hydroxy- 3-methyl-but-2-enyl pyrophosphate (HMBPP, 1 μM). After incubation, we analyzed the activation state of γδ T cells by measuring the expression of CD69 and perforin by flow cytometry, and TNF-alpha and IFN-gamma production by ELISA.Our results indicated that HMBPP-stimulated γδ T cells exhibit an increase in CD69 expression (p