IMEX   05356
INSTITUTO DE MEDICINA EXPERIMENTAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
DECIDUALIZATION PROCESS CONDITIONS MYELOID REGULATORY CELLS
Autor/es:
ELIZABETH SOCZEWSKI; ANA SCHAFIR; CLAUDIA PÉREZ LEIRÓS; SOLEDAD GORI; LAURA FERNÁNDEZ; ALEJANDRA GRASSI BASSINO; ROSANNA RAMHORST; ESTEBAN GRASSO; JORGELINA BLEJER; GABRIELA SALAMONE
Lugar:
Tucumán
Reunión:
Congreso; Sociedad Argentina Inmunología; 2019
Institución organizadora:
Sociedad Argentina de Inmunología
Resumen:
DC-10 is a novel and distinctive subset of tolerogenic dendritic cell (DC) characterized to be a potent IL-10 producer and type 1 Tregs (Tr1)-inducer. Although DC-10 and others subsets of Myeloid regulatory cells (Mregs) were recently found increased at the human decidua, their role in materno-fetal tolerance maintenance are still unknown. Due to decidualization process involves immunoregulatory factors secretion by endometrial stromal cells, we focus on the impact of this process in conditioning monocytes to Mregs subsets. For this, conditioned media (CM) of Human endometrial stromal cell line (HESC) decidualized with MPA+dbcAMP for 8 days (Dec-CM) or not (Non-dec CM) were collected. Then, isolated monocytes from peripheral blood mononuclear cells of healthy donors were cultured with rhGM-CSF+rhIL-4 for 5 days in the absence/presence of CM. We observed that Dec-CM was able to inhibit CD14+ cells differentiation to immature DC (p