IMEX   05356
INSTITUTO DE MEDICINA EXPERIMENTAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Polymorphism in Dectin-1 and tuberculosis disease
Autor/es:
SCARFO MARINA; MONICA CUFRE; ALEJANDRA DUARTE; IGNACIO MARTIN; MERCEDES ALEMAN; MERCEDES PASTORINI; LEIVA CAROLINA
Lugar:
Mar del Plata
Reunión:
Congreso; Reunión conjunta de Sociedades de Biociencias; 2019
Resumen:
Tuberculosis (TB) infection is determined by the complex interaction between Mycobacterium tuberculosis (Mtb) and host genetic factors. We have demonstrated that the simultaneous binding of Mtb with Dectin-1 and TLR2 induces the generation of ROS in neutrophils. Until today, those studies of the implication of SNP mutations in Mtb receptors have been variable except for TLR2 which effect on TB susceptibility is indisputable. The relationship of polymorphism in Dectin-1 with tuberculous disease has not been studied until today. A single nucleotide functional polymorphism (SNP) in Dectin-1 (Y238X, rs16910526) generates an early stop codon, resulting in the loss of the last 10 amino acids of the carbohydrate recognition domain which was associated with recurrent cutaneous mycoses, and reflected in a lower membrane expression. The aim of this work was to evaluate the frequency of polymorphism in the population, the possible association with TB and tests in vitro. Results: The polymorphism Y238X, rs16910526 was not present in the total population studied (Healthy Subject, HS, n=25 and TB, n= 25). On the other hand TB patients showed a significant increase in the total GB cell count (p< 0.01) and also the percentage of neutrophils (p