IMEX   05356
INSTITUTO DE MEDICINA EXPERIMENTAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
HUMAN NEUTROPHILS RESPONSES TO RESPIRATORY SYNCYTIAL VIRUS STIMULATION
Autor/es:
SALINAS M; BAUMEISTER, ELSA; GEFFNER, JORGE RAÚL; SABBIONE, FLORENCIA; SHIROMIZU, CAROLINA MAIUMI; MIGLIO RODRIGUEZ, MAXIMILIANO S.; PONTORIERO, ANDREA; JANCIC, CAROLINA C.; TREVANI, ANALÍA S.; KEITELMAN, IRENE A.; FUENTES, FEDERICO; RUSSO, MARA; BUENO, CARLOS
Lugar:
Tucumán
Reunión:
Congreso; LXVII Reunión científica anual de la Sociedad Argentina de Inmunología; 2019
Institución organizadora:
SAI
Resumen:
Respiratory Syncytial Virus (RSV) infections represent the leading cause of bronchiolitis in infants and young children worldwide and are responsible for almost 200000 deaths annually.Neutrophils constitute the predominant cellular infiltrate in the airways of infants with RSVbronchiolitis.Previous studies indicated that RSV induces Neutrophil Extracellular Traps(NETs). The aim of this study was to further investigate the response of highly purified human neutrophils to RSV. In agreement with previous studies, we determined by immunostaining and confocal microscopy that purified RSV induces NETosis (n=4). By quantification of DNA inculture supernatants, we detected that neutrophils produce NETs in a wide range of multiplicity of infections (n=4). However, we did not detect significant differences in LDH activity in culture supernatants at distinct MOIs evaluated (n=6). These findings suggest that only a small proportion of neutrophils undergoes lytic NETosis, something which was also observed by timelapselive microscopy with NucSpot 488, a cell-permeable DNA-dye (n: 1). In this livemicroscopy preliminary assay, we observed the appearance of extracellular vesicles in culture supernatants, which progressively increased by 4 h p.s. Thus, we isolated these neutrophil derived-EVs (EV) and evaluated their impact on human monocytes isolated by adherence. RSVinduced EV (produced by 106 neutrophils) promoted monocyte IL-6 release in contrast to EV produced by unstimulated neutrophils (868±139 vs 232±84 pg/ml; p