ROLE OF IL10 ON MYELOID CELLS EXPANSION DURING THE IMMUNOSUPPRESSION INDUCED BY BACTERIAL ENDOTOXINS

DANIELA MONTAGNA; ADRIANA FONTANALS; JULIETA ALCAIN; GASTÓNDOMINGUEZ; MARIA FLORENCIA TODERO; MONICA VERMEULEN; BÁRBARA REARTE

Tucumán

Congreso; LVII REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INMUNOLOGÍA (SAI).; 2019

In sepsis caused by Gram-negative infections, endotoxin LPS induces an immunosuppression (IS) state in which IL-10 participates. Myeloid cells are a heterogeneous population that expands in pathological conditions. We aim was to evaluate the phenotypic and functional aspects of myeloid cells and its relationship with IL-10 for the understanding of the IS processes. For this, BALB /c wild type (WT) and IL-10 deficient (KO) mice were used. IS context was obtained with a regimen of increasing doses of LPS (0.25-10ug/mouse) for 10 days (WTIS and KOIS). Population determinations: flow cytometry. Respiratory burst: DHR. Proliferation assays: CFSE. In peripheral blood KOIS mice showed a significant increase in myeloid cells (CD11b+GR1+ (N° abs): WT =1.5x108±5.7x107; WTIS =2.3x108±5.7x107; KO =1.9x108±5.7x107; KOIS =3.2x108±1.6x108*, *p