Molecular involvement of IL10 SNP rs1800896 genotypes and IL-10 cytokine expression in the clinical severity of Hemolytic Uremic Syndrome.

GONZALO PINEDA,; SANTIAGO, ADRIANA; EXENI, RAMON; MARÍA VICTORIA RAMOS,; MONGELOS, MICAELA; BRUBALLA, ANDREA; ROSSETTI, LILIANA; PALERMO M; FIORENTINO, GABRIELA ALEJANDRA; ABELLEYRO, MARTIN; DE BRASI CARLOS

Buenos Aires

Congreso; Reunion de Sociedades de Biociencias. LXVIII Reunión Científica Anual de la Sociedad Argentina de Inmunología (SAI). LXV Reunión Anual De La Sociedad Argentina De Investigación Clínica (SAIC).; 2020

Title: Molecular involvement of IL10 SNP rs1800896 genotypes and IL-10 cytokine expression in the clinical severity of Hemolytic Uremic Syndrome.Micaela Mongelos1, Gonzalo Pineda1, Gabriela Fiorentino2, Andrea Bruballa1, Adriana Santiago2, Martín Abelleyro3, Liliana Rossetti3, Ramón Exeni2, Carlos De Brasi3, Marina Palermo1, María Victoria Ramos1.1Patogénesis E Inmunología De Procesos Infecciosos, IMEX. 2Hospital del Niño, San Justo, La Matanza. 3Genética Molecular de la Hemofilia, IMEX.Introduction: Although Hemolytic Uremic Syndrome (HUS) is caused by Shiga toxin (Stx)-producing E. coli, the inflammatory response is essential for disease progression. Despite antiinflammatory mechanisms are triggered, studies assessing the implication of such mediators, like IL-10, in HUS are scarce. Interindividual differences in IL-10 levels are related to genetic variants. Several reports have linked the single nucleotide polymorphism (SNP) rs1800896 (Legacy: -1082A>G), located in the IL10 promoter, to several diseases. Objective: The aim of this work was to evaluate the contribution of IL-10 and SNP -1082A>G to HUS severity. Methods: Blood samples were collected from HUS patients at the acute period and from healthy children (HC). Plasma was stored and mononuclear cells (PBMC) were isolated and cultured with Medium or LPS for 20h. IL-10 levels were evaluated in plasma and PBMC supernatants by ELISA. DNA was obtained from PBMC and IL10 -1082A>G analysis was performed by allele specific-PCR. Results: Circulating IL-10 levels were increased in HUS (Mean±SEM (pg/mL): HUS=60.9±10.0***; HC=9.3±2.1; n=18,17; ***p