IMEX   05356
INSTITUTO DE MEDICINA EXPERIMENTAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Neutrophil autopaghy during human active tuberculosis is modulated by SLAMF1
Autor/es:
PELLEGRINI, JOAQUÍN M.; TATEOSIAN, NANCY L.; PALMERO, DJ; TREVANI AS; SABBIONE F; CASTELLO, FA; LEVI, A; GARCIA, VE; MORELLI, MARÍA PAULA; AMIANO, N; CIALLELLA, L
Lugar:
Tucumán
Reunión:
Congreso; LXVII Reunión Anual de la Sociedad Argentina de Inmunología; 2019
Resumen:
BACKGROUND: Neutrophils infected with Mycobacterium tuberculosis (Mtb) predominatein patient´s lungs. Neutrophilic infiltration represents a characteristic feature of severetuberculosis (TB) but neutrophil biology during TB remains poorly understood. Autophagy iscrucial for several neutrophil functions and can be modulated by different mediators. Thecostimulator SLAMF1 acts as a microbial sensor in macrophages but its expression inhuman neutrophils has not been evaluated.METHODS: We investigated whether SLAMF1 participates in neutrophil autophagy againstMtb. Neutrophils were isolated from heparinized blood from TB patients and healthy donorsand cultured (2x106 cells/ml) with Mtb-Ag (10µg/ml) ± SLAMF1 mAb (10µg/ml) during 2 h.Some experiments were performed in the presence of a NADPH-oxidase inhibitor. SLAMF1and autophagy levels were analyzed by flow cytometry and confocal microscopy. P valuesof